High-throughput screening approaches to identify regulators of mammalian autophagy

被引：10

作者：

Joachim, Justin ^{[1
]}

Jiang, Ming ^{[2
]}

McKnight, Nicole C. ^{[3
]}

Howell, Michael ^{[2
]}

Tooze, Sharon A. ^{[1
]}

机构：

[1] Canc Res UK, London Res Inst, Secretory Pathway Lab, London WC2A 3LY, England

[2] Canc Res UK, London Res Inst, High Throughput Screening Unit, London WC2A 3LY, England

[3] Icahn Sch Med Mt Sinai, Dept Neurol, Hess Ctr Sci & Med, New York, NY 10029 USA

来源：

METHODS | 2015年 / 75卷

关键词：

Autophagy; Autophagosome; High-throughput screens; siRNA; Drug and compound libraries; Amino acid starvation; GFP-LC3; p62; Z-score; REVEALS; HOMOLOG; DISEASE; ASSAYS;

D O I：

10.1016/j.ymeth.2015.02.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This article discusses the issues to consider in the development and implementation of high-throughput screens (HTSs) using both siRNA libraries and small molecule compound collections, in order to discover autophagy regulators in mammalian cells. We discuss how to develop the screen, focusing on the key parameters to establish in order to perform a successful screen. As our understanding of autophagy increases and its impact on human disease is elucidated, this technology can be further exploited to uncover novel genes, which may one day become new therapeutic targets. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：96 / 104

页数：9

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