Expression of transforming growth factor β1 and transforming growth factor β type-II receptor mRNA in papillary thyroid carcinoma

Transforming growth factor-beta(1) (TGF-beta(1)) is a potent inhibitor of epithelial cell proliferation. Signal transduction by TGF-beta(1) involves direct binding to the TGF-beta Type-II receptor and then the formation of a heterodimeric complex of TGF-beta Type-I and Type-Il receptor. To explore the role of TGF-beta(1) in thyroid carcinoma, we examined the expression of TGF-beta(1) and TGF-beta Type-II receptor mRNA by northern blotting analysis in both 14 papillary thyroid carcinomas and surrounding normal thyroid tissues. Relative mRNA level was determined by scanning densitometry of the autoradiogram and corrected for loading differences using a human p-actin cDNA probe. The relative mRNA levels of TGF-beta(1), in 12 out of 14 papillary thyroid carcinomas were higher than those in surrounding normal thyroid tissues. In contrast, the relative mRNA levels of TGF-beta Type-II receptor were reduced to 60.1 +/- 18.3% of those of normal thyroid tissues in 10 papillary thyroid carcinomas. There were no clear relationships between the relative mRNA levels for TGF-beta, and TGF-beta Type-II receptor and the histological characteristics of papillary thyroid carcinomas. The relative mRNA levels for TGF-beta, and TGF-beta Type-II receptor did not show significant differences in thyroid carcinomas with or without lymph node metastases. There was a negative correlation between the TGF-beta Type-II receptor mRNA level and tumor size, while no significant correlation was observed between the TGF-beta(1) mRNA level and tumor size. In conclusion, most papillary thyroid carcinomas overexpress TGF-beta(1) mRNA but exhibit a reduction in TGF-beta Type-II receptor mRNA. The reduction of TGF-beta Type-II receptor mRNA may play a role in the pathogenesis of papillary thyroid carcinoma.