TAR cloning and integrated overexpression of 6-demethylchlortetracycline biosynthetic gene cluster in Streptomyces aureofaciens

被引：16

作者：

Wu, Naxin ^{[1
,2
]}

Huang, He ^{[3
]}

Min, Taoling ^{[2
]}

Hu, Haifeng ^{[2
]}

机构：

[1] Fudan Univ, Sch Pharm, Dept Pharmacol, Shanghai 201203, Peoples R China

[2] Shanghai Inst Pharmaceut Ind, Dept Biopharmceut, Shanghai 201203, Peoples R China

[3] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Key Lab Synthet Biol, Shanghai 200032, Peoples R China

来源：

ACTA BIOCHIMICA ET BIOPHYSICA SINICA | 2017年 / 49卷 / 12期

基金：

上海市自然科学基金;

关键词：

6-demethylchlortetracycline; Streptomyces aureofaciens; biosynthetic gene cluster; transformation-associated recombination; RECOMBINATION CLONING; SELECTIVE ISOLATION; HETEROLOGOUS EXPRESSION; COMPLEX GENOMES; DNA; BIOLOGY; COLI;

D O I：

10.1093/abbs/gmx110

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

6-Demethylchlortetracycline (6-DCT), a tetracycline antibiotic produced by Streptomyces aureofaciens, is a crucial precursor employed for the semi-synthesis of tigecycline, minocycline, and amadacyclin (PTK 0796). In this study, the 6-DCT biosynthetic gene cluster (BGC) was cloned from genomic DNA of a high 6-DCT-producing strain, S. aureofaciens DM-1, using the transformation-associated recombination method. An extra copy of the 6-DCT BGC was introduced and integrated into the chromosome of S. aureofaciens DM-1. Duplication of the 6-DCT BGC resulted in a maximum increase of the 6-DCT titer by 34%.

引用

页码：1129 / 1134

页数：6

共 50 条

[31] Cloning and Heterologous Expression of the Grecocycline Biosynthetic Gene Cluster
Bilyk, Oksana
Sekurova, Olga N.
Zotchev, Sergey B.
Luzhetskyy, Andriy
PLOS ONE, 2016, 11 (07):
[32] Cloning and identification of the lobophorin biosynthetic gene cluster from marine Streptomyces olivaceus strain FXJ7.023
Yue, Changwu
Niu, Jing
Liu, Ning
Lu, Yuhong
Liu, Minghao
Li, Yuanyuan
Wang, Miao
Shao, Meiyun
Qian, Shenyan
Bao, Yuxin
Huang, Ying
PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2016, 29 (01) : 287 - 293
[33] CLONING OF THE PUTATIVE GLYCOGEN BRANCHING ENZYME GENE, GLGB, FROM STREPTOMYCES-AUREOFACIENS
HOMEROVA, D
KORMANEC, J
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1994, 1200 (03): : 334 - 336
[34] Precise cloning and tandem integration of large polyketide biosynthetic gene cluster using Streptomyces artificial chromosome system
Hee-Ju Nah
Min-Woo Woo
Si-Sun Choi
Eung-Soo Kim
Microbial Cell Factories, 14
[35] Cloning, reassembling and integration of the entire nikkomycin biosynthetic gene cluster into Streptomyces ansochromogenes lead to an improved nikkomycin production
Liao, Guojian
Li, Jine
Li, Lei
Yang, Haihua
Tian, Yuqing
Tan, Huarong
MICROBIAL CELL FACTORIES, 2010, 9
[36] Cloning, reassembling and integration of the entire nikkomycin biosynthetic gene cluster into Streptomyces ansochromogenes lead to an improved nikkomycin production
Guojian Liao
Jine Li
Lei Li
Haihua Yang
Yuqing Tian
Huarong Tan
Microbial Cell Factories, 9
[37] Precise cloning and tandem integration of large polyketide biosynthetic gene cluster using Streptomyces artificial chromosome system
Nah, Hee-Ju
Woo, Min-Woo
Choi, Si-Sun
Kim, Eung-Soo
MICROBIAL CELL FACTORIES, 2015, 14
[38] Characterization of the biosynthetic gene cluster for cryptic phthoxazolin A in Streptomyces avermitilis
Suroto, Dian Anggraini
Kitani, Shigeru
Arai, Masayoshi
Ikeda, Haruo
Nihira, Takuya
PLOS ONE, 2018, 13 (01):
[39] Characterization of the Biosynthetic Gene Cluster for the Antibiotic Armeniaspirols in Streptomyces armeniacus
Qiao, Yongjian
Yan, Jiayan
Jia, Jia
Xue, Jiao
Qu, Xudong
Hu, Yunfeng
Deng, Zixin
Bi, Hongkai
Zhu, Dongqing
JOURNAL OF NATURAL PRODUCTS, 2019, 82 (02): : 318 - 323
[40] The biosynthetic gene cluster for the β-lactam carbapenem thienamycin in Streptomyces cattleya
Núñez, LE
Méndez, C
Braña, AF
Blanco, G
Salas, JA
CHEMISTRY & BIOLOGY, 2003, 10 (04): : 301 - 311

← 1 2 3 4 5 →