TAR cloning and integrated overexpression of 6-demethylchlortetracycline biosynthetic gene cluster in Streptomyces aureofaciens

被引：16

作者：

Wu, Naxin ^{[1
,2
]}

Huang, He ^{[3
]}

Min, Taoling ^{[2
]}

Hu, Haifeng ^{[2
]}

机构：

[1] Fudan Univ, Sch Pharm, Dept Pharmacol, Shanghai 201203, Peoples R China

[2] Shanghai Inst Pharmaceut Ind, Dept Biopharmceut, Shanghai 201203, Peoples R China

[3] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Key Lab Synthet Biol, Shanghai 200032, Peoples R China

来源：

ACTA BIOCHIMICA ET BIOPHYSICA SINICA | 2017年 / 49卷 / 12期

基金：

上海市自然科学基金;

关键词：

6-demethylchlortetracycline; Streptomyces aureofaciens; biosynthetic gene cluster; transformation-associated recombination; RECOMBINATION CLONING; SELECTIVE ISOLATION; HETEROLOGOUS EXPRESSION; COMPLEX GENOMES; DNA; BIOLOGY; COLI;

D O I：

10.1093/abbs/gmx110

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

6-Demethylchlortetracycline (6-DCT), a tetracycline antibiotic produced by Streptomyces aureofaciens, is a crucial precursor employed for the semi-synthesis of tigecycline, minocycline, and amadacyclin (PTK 0796). In this study, the 6-DCT biosynthetic gene cluster (BGC) was cloned from genomic DNA of a high 6-DCT-producing strain, S. aureofaciens DM-1, using the transformation-associated recombination method. An extra copy of the 6-DCT BGC was introduced and integrated into the chromosome of S. aureofaciens DM-1. Duplication of the 6-DCT BGC resulted in a maximum increase of the 6-DCT titer by 34%.

引用

页码：1129 / 1134

页数：6

共 50 条

[1] Mechanism of the incidental production of a melanin-like pigment during 6-demethylchlortetracycline production in Streptomyces aureofaciens
Nakano, T
Miyake, K
Ikeda, M
Mizukami, T
Katsumata, R
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (04) : 1400 - 1404
[2] Cloning and characterization of a new polyketide synthase gene cluster in Streptomyces aureofaciens CCM 3239
Novakova, R
Bistakova, J
Homerova, D
Rezuchova, B
Feckova, L
Kormanec, J
DNA SEQUENCE, 2004, 15 (03): : 188 - 195
[3] Cloning and analysis of the simocyclinone biosynthetic gene cluster of Streptomyces antibioticus TO 6040
Galm, U
Schimana, J
Fiedler, HP
Schmidt, J
Li, SM
Heide, L
ARCHIVES OF MICROBIOLOGY, 2002, 178 (02) : 102 - 114
[4] Cloning of tunicamycin biosynthetic gene cluster from Streptomyces chartreusis NRRL 3882
Suman Karki
So-Yeon Kwon
Hyung-Jin Kwon
Journal of the Korean Society for Applied Biological Chemistry, 2011, 54 : 136 - 140
[5] Cloning of Tunicamycin Biosynthetic Gene Cluster from Streptomyces chartreusis NRRL 3882
Karki, Suman
Kwon, So-Yeon
Kwon, Hyung-Jin
JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY, 2011, 54 (01): : 136 - 140
[6] Molecular cloning and identification of the laspartomycin biosynthetic gene cluster from Streptomyces viridochromogenes
Wang, Yang
Chen, Ying
Shen, Qirong
Yin, Xihou
GENE, 2011, 483 (1-2) : 11 - 21
[7] Cloning and analysis of the simocyclinone biosynthetic gene cluster of Streptomyces antibioticus Tü 6040
Ute Galm
Judith Schimana
Hans-Peter Fiedler
Jürgen Schmidt
Shu-Ming Li
Lutz Heide
Archives of Microbiology, 2002, 178 : 102 - 114
[8] Cloning and functional analysis of the naphthomycin biosynthetic gene cluster in Streptomyces sp CS
Wu, Yingying
Kang, Qianjin
Shen, Yuemao
Su, Wenjin
Bai, Linquan
MOLECULAR BIOSYSTEMS, 2011, 7 (08) : 2459 - 2469
[9] Cloning and heterologous expression of the spectinabilin biosynthetic gene cluster from Streptomyces spectabilis
Choi, Yoo Seong
Johannes, Tyler W.
Simurdiak, Michael
Shao, Zengyi
Lu, Haige
Zhao, Huimin
MOLECULAR BIOSYSTEMS, 2010, 6 (02) : 336 - 338
[10] Cloning of an avilamycin biosynthetic gene cluster from Streptomyces viridochromogenes Tu57
Gaisser, S
Trefzer, A
Stockert, S
Kirschning, A
Bechthold, A
JOURNAL OF BACTERIOLOGY, 1997, 179 (20) : 6271 - 6278

← 1 2 3 4 5 →