Preliminary time-of-flight neutron diffraction study of human deoxyhemoglobin

被引：10

作者：

Kovalevsky, A. Y. ^{[3
]}

Chatake, T. ^{[1
]}

Shibayama, N. ^{[2
]}

Park, S. -Y. ^{[4
]}

Ishikawa, T. ^{[1
]}

Mustyakimov, M. ^{[3
]}

Fisher, S. Z. ^{[3
]}

Langan, P. ^{[3
,5
]}

Morimoto, Y. ^{[1
]}

机构：

[1] Kyoto Univ, Inst Res Reactor, Osaka 5900494, Japan

[2] Jichi Med Univ, Dept Physiol, Shimotsuke, Tochigi 3290498, Japan

[3] Los Alamos Natl Lab, Biosci Div, Los Alamos, NM 87545 USA

[4] Yokohama City Univ, Grad Sch Integrated Sci, Yokohama, Kanagawa 2300045, Japan

[5] Univ Toledo, Dept Chem, Toledo, OH 43606 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2008年 / 64卷

关键词：

D O I：

10.1107/S1744309108005137

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Human hemoglobin ( HbA) is an intricate system that has evolved to efficiently transport oxygen molecules ( O-2) from lung to tissue. Its quaternary structure can fluctuate between two conformations, T ( tense or deoxy) and R ( relaxed or oxy), which have low and high affinity for O2, respectively. The binding of O2 to the heme sites of HbA is regulated by protons and by inorganic anions. In order to investigate the role of the protonation states of protein residues in O2 binding, large crystals of deoxy HbA ( similar to 20 mm(3)) were grown in D2O under anaerobic conditions for neutron diffraction studies. A time-of-flight neutron data set was collected to 1.8 angstrom resolution on the Protein Crystallography Station ( PCS) at the spallation source run by Los Alamos Neutron Science Center ( LANSCE). The HbA tetramer ( 64.6 kDa; 574 residues excluding the four heme groups) occupies the largest asymmetric unit ( space group P2(1)) from which a high-resolution neutron data set has been collected to date.

引用

页码：270 / 273

页数：4

共 50 条

[41] Inelasticity corrections for time-of-flight and fixed wavelength neutron diffraction experiments
Soper, A. K.
MOLECULAR PHYSICS, 2009, 107 (16) : 1667 - 1684
[42] SINGLE-CRYSTAL TIME-OF-FLIGHT NEUTRON-DIFFRACTION AT IPNS
SCHULTZ, AJ
LEUNG, P
WILLIAMS, JM
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1983, 185 (MAR): : 3 - INOR
[43] Stroboscopic time-of-flight neutron diffraction in long pulsed magnetic fields
Nakajima, Taro
Watanabe, Masao
Inamura, Yasuhiro
Matsui, Kazuki
Kanda, Tomoki
Nomoto, Tetsuya
Ohishi, Kazuki
Kawamura, Yukihiko
Saito, Hiraku
Tamatsukuri, Hiromu
Terada, Noriki
Kohama, Yoshimitsu
PHYSICAL REVIEW RESEARCH, 2024, 6 (02):
[44] Materials Structure and Strain Analysis Using Time-of-flight Neutron Diffraction
Zhang, Shu Yan
Godfrey, Evelyn
Abbey, Brian
Xu, Pingguang
Tomota, Yo
Liljedahl, David
Zanellato, Olivier
Fitzpatrick, Michael
Kelleher, Joe
Siano, Salvatore
Santisteban, Javier
Korsunsky, Alexander M.
WORLD CONGRESS ON ENGINEERING 2009, VOLS I AND II, 2009, : 1412 - +
[45] Strain investigations on calcite marbles using neutron time-of-flight diffraction
Scheffzük, C
Siegesmund, S
Koch, A
ENVIRONMENTAL GEOLOGY, 2004, 46 (3-4): : 468 - 476
[46] THE STRUCTURE OF SRRUO3 BY TIME-OF-FLIGHT NEUTRON POWDER DIFFRACTION
JONES, CW
BATTLE, PD
LIGHTFOOT, P
HARRISON, WTA
ACTA CRYSTALLOGRAPHICA SECTION C-CRYSTAL STRUCTURE COMMUNICATIONS, 1989, 45 : 365 - 367
[47] Single-Crystal Time-of-Flight Neutron Diffraction: IPNS to SNS
Schultz, Arthur
ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES, 2022, 78 : A73 - A73
[48] DETERMINATION OF THE VELOCITY OF SOUND IN CRYSTALS BY TIME-OF-FLIGHT NEUTRON-DIFFRACTION
WILLIS, BTM
ACTA CRYSTALLOGRAPHICA SECTION A, 1986, 42 : 514 - 525
[49] A system of detection and data acquisition for time-of-flight neutron diffraction setups
Marin, V. N.
Potashev, S. I.
Trunov, D. N.
Potashev, A. S.
Aksenov, S. N.
Afonin, A. A.
Litvin, V. S.
Alexeev, A. A.
Kononykhin, A. S.
Sadykov, R. A.
INSTRUMENTS AND EXPERIMENTAL TECHNIQUES, 2014, 57 (06) : 684 - 687
[50] The birth of time-of-flight (TOF) neutron powder diffraction at pulsed neutron source (invited)
Sosnowska, Izabela M.
CRYSTAL RESEARCH AND TECHNOLOGY, 2015, 50 (9-10) : 705 - 715

← 1 2 3 4 5 →