Analysis of the function of the photoreceptors phytochrome B and phytochrome D in Nicotiana plumbaginifolia and Arabidopsis thaliana

被引：10
|
作者：
Fernández, AP
Gil, P
Valkai, I
Nagy, F
Schäfer, E
机构：
[1] Univ Freiburg, Ints Biol Bot 2, D-79104 Freiburg, Germany
[2] Biol Res Ctr, Inst Plant Biol, H-6726 Szeged, Hungary
来源：
PLANT AND CELL PHYSIOLOGY | 2005年 / 46卷 / 05期
关键词：
light signalling; Nicotiana plumbaginifolia; phytochrome;
D O I：
10.1093/pcp/pci073
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
To investigate the mechanism of phytochrome action in vivo, NtPHYB, AtPHYB and phyD:green fluorescent protein (GFP) were overexpressed in Nicotiana plumbaginifolia and Arabidopsis thaliana. The expression of 35S:NtPHYB:GFP and 35S:AtPHYB:GFP complemented the tobacco hgl2 and Arabidopsis phyB-9 mutations, whereas the 35S:AtPHYD:GFP only rescued the hgl2 mutant. All three fusion proteins are transported into the nucleus in all genetic backgrounds. These data indicate that AtPHYD:GFP is biologically active and functions as the main red light receptor in transgenic tobacco, and establish an experimental system for the functional analysis of this elusive photoreceptor in vivo.
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页码：790 / 796
页数：7
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