Analysis of the function of the photoreceptors phytochrome B and phytochrome D in Nicotiana plumbaginifolia and Arabidopsis thaliana

被引：10
|
作者：
Fernández, AP
Gil, P
Valkai, I
Nagy, F
Schäfer, E
机构：
[1] Univ Freiburg, Ints Biol Bot 2, D-79104 Freiburg, Germany
[2] Biol Res Ctr, Inst Plant Biol, H-6726 Szeged, Hungary
来源：
PLANT AND CELL PHYSIOLOGY | 2005年 / 46卷 / 05期
关键词：
light signalling; Nicotiana plumbaginifolia; phytochrome;
D O I：
10.1093/pcp/pci073
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
To investigate the mechanism of phytochrome action in vivo, NtPHYB, AtPHYB and phyD:green fluorescent protein (GFP) were overexpressed in Nicotiana plumbaginifolia and Arabidopsis thaliana. The expression of 35S:NtPHYB:GFP and 35S:AtPHYB:GFP complemented the tobacco hgl2 and Arabidopsis phyB-9 mutations, whereas the 35S:AtPHYD:GFP only rescued the hgl2 mutant. All three fusion proteins are transported into the nucleus in all genetic backgrounds. These data indicate that AtPHYD:GFP is biologically active and functions as the main red light receptor in transgenic tobacco, and establish an experimental system for the functional analysis of this elusive photoreceptor in vivo.
引用
收藏
页码：790 / 796
页数：7
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