Detergents enhance EspB secretion from Escherichia coli strains harboring the locus for the enterocyte effacement (LEE) gene

被引：4
|
作者：
Nakasone, Noboru [1 ]
Toma, Claudia [1 ]
Higa, Naomi [1 ]
Koizumi, Yukiko [1 ]
Ogura, Yasunori [1 ]
Suzuki, Toshihiko [1 ]
机构：
[1] Univ Ryukyus, Grad Sch Med, Dept Mol Bacteriol & Immunol, Okinawa 9030125, Japan
来源：
FEMS MICROBIOLOGY LETTERS | 2011年 / 315卷 / 02期
关键词：
EspB secretion; detergent enhancement; EPEC and STEC; PROTEIN SECRETION; BILE-ACIDS; EXPRESSION;
D O I：
10.1111/j.1574-6968.2010.02176.x
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The effects of detergents (cholic acid, deoxycholic acid, Triton X-100, and Nonidet P-40) on the secretion of EspB from the locus for enterocyte effacement (LEE) gene-positive Escherichia coli strains were examined. Clinical isolates of eight EPEC strains and seven STEC strains were used to detect EspB after they had been cultivated in Luria-Bertani (LB) broth containing one of the detergents. When the bacteria were cultured in LB broth supplemented with one of the detergents, the amount of EspB produced was increased by 2-32-fold depending on the detergent and the strain used. EspB was detected in all strains when they were cultured in LB broth containing all of the detergents. The results obtained in this study can be applied to immunological diagnostic methods for detecting EspB and also to the production of EspB for research purposes.
引用
收藏
页码：109 / 114
页数：6
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