Molecular cloning of rabbit matrix metalloproteinase-2 and its broad expression at several tissues

被引：11
|
作者：
Matsumoto, S
Katoh, M
Watanabe, T
Masuho, Y
机构：
[1] UNIV TSUKUBA,INST BASIC MED SCI,TSUKUBA,IBARAKI 305,JAPAN
[2] YAMANOUCHI PHARMACEUT CO LTD,INST DRUG DISCOVERY RES,MOLEC MED RES LAB,TSUKUBA,IBARAKI 305,JAPAN
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1996年 / 1307卷 / 02期
关键词：
dideoxy sequencing; PCR; northern blot analysis; homology; gelatinase; rabbit;
D O I：
10.1016/0167-4781(96)00050-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have cloned cDNA encoding rabbit matrix metalloproteinase-2 (MMP-2, 72 kDa type IV collagenase) by a combination of conventional library screening, the 'single strand ligation to single-stranded cDNA (SLIC)' method and 'long and accurate PCR (LA-PCR)'. Deduced amino acid sequence was highly conserved through mammalian species. Northern blot analysis revealed that rabbit MMP-2 had 2 species of mRNA, 2.8 kbp and 3.5 kbp, and were expressed constitutively in all the tissues tested. This was totally different from mRNA expression of rabbit MMP-1, -3 and -9.
引用
收藏
页码：137 / 139
页数：3
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