The purification of IgY from chicken egg yolk by preparative electrophoresis

被引：21
|
作者：
Gee, SC [1 ]
Bate, IM [1 ]
Thomas, TM [1 ]
Rylatt, DB [1 ]
机构：
[1] Gradipore Ltd, Frenchs Forest, NSW 2086, Australia
来源：
PROTEIN EXPRESSION AND PURIFICATION | 2003年 / 30卷 / 02期
关键词：
D O I：
10.1016/S1046-5928(03)00076-7
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Chicken IgY has been purified from egg yolk by preparative electrophoresis on the Gradiflow, a system which has been employed for the purification of a wide range of proteins with high recovery and biological activity. Protein purification on the Gradiflow utilises electrophoresis with selected combinations of porous membranes and buffers. The purification of IgY was achieved by initial PEG lipid precipitation, then a single step Gradiflow run by a strategy based on the relatively high pI range of IgY compared to other egg yolk proteins. The IgY yields obtained from the delipidised supernatant are consistently greater than 80% by immunoassay. The purity of the IgY fraction compared favourably with IgY prepared using three commercial products. (C) 2003 Elsevier Science (USA). All rights reserved.
引用
收藏
页码：151 / 155
页数：5
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