Amplified fragment length polymorphism analyses of Eimeria spp.:: an improved process for genetic studies of recombinant parasites

被引：29

作者：

Blake, DP ^{[1
]}

Smith, AL ^{[1
]}

Shirley, MW ^{[1
]}

机构：

[1] Inst Anim Hlth, Compton RG20 7NN, Berks, England

来源：

PARASITOLOGY RESEARCH | 2003年 / 90卷 / 06期

关键词：

D O I：

10.1007/s00436-003-0890-x

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Application of the amplified fragment length polymorphism (AFLP) technique to genetic mapping studies requires high quality DNA as a template. In the case of Eimeria spp., this has previously been in the form of chromosomal DNA obtained from purified sporozoites recovered from large numbers of oocysts (generally up to 2x10(8)). In order for the AFLP technique to be more easily applied to studies on the genetics of Eimeria maxima, for which only smaller numbers of oocysts are available, a simplified, more efficient method for the recovery of genomic DNA from small numbers of oocysts was developed. Our new method should also be useful for genetic analyses of other coccidial parasites and for the recovery of AFLP-quality DNA from other pathogens.

引用

页码：473 / 475

页数：3

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