qRT-PCR Platforms for Diagnosing and Reporting SARS- CoV-2 Infection in Human Samples

被引：0
|
作者：
Giadone, Richard M. [1 ,2 ]
Mithal, Aditya [1 ,2 ]
Miller, Gregory J. [1 ,2 ]
Matte, Taylor M. [1 ,2 ]
Yeung, Anthony K. [1 ,2 ]
Dowrey, Todd W. [1 ,2 ]
Werder, Rhiannon B. [1 ,2 ]
Miller, Nancy S. [4 ,5 ]
Andry, Christopher D. [4 ,5 ]
Vanuytsel, Kim [1 ,2 ,3 ]
Murphy, George J. [1 ,2 ,3 ]
机构：
[1] Boston Univ, Ctr Regenerat Med, Boston, MA 02118 USA
[2] Boston Med Ctr, Boston, MA 02118 USA
[3] Boston Univ, Sch Med, Dept Med, Sect Hematol & Oncol, Boston, MA 02218 USA
[4] Boston Med Ctr, Dept Pathol & Lab Med, Boston, MA 02218 USA
[5] Boston Univ, Sch Med, Boston, MA 02218 USA
来源：
STAR PROTOCOLS | 2020年 / 1卷 / 02期
关键词：
D O I：
10.1016/j.xpro.2020.100102
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The protocols herein outline the use of qRT-PCR to detect the presence of SARSCoV-2 genomic RNA in patient samples. In order to cope with potential fluctuations in supply chain and testing demands and to enable expedient adaptation of reagents and assays on hand, we include details for three parallel methodologies (one-and two-step singleplex and one-step multiplex assays). The diagnostic platforms described can be easily adapted by basic science research laboratories for SARS-CoV-2 diagnostic testing with relatively short turnaround time. For complete details on the use and execution of this protocol, please refer to Vanuytsel et al. (2020).
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页数：19
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