An Improved Protocol for the Production of Lentiviral Vectors

被引：20
|
作者：
Brown, Logan Y. [1 ]
Dong, Wendy [1 ]
Kantor, Boris [1 ,2 ]
机构：
[1] Duke Univ, Dept Neurobiol, Duke Viral Vector Core, Med Ctr, Durham, NC 27710 USA
[2] CLAIRIgene Inc, Durham, NC 27701 USA
来源：
STAR PROTOCOLS | 2020年 / 1卷 / 03期
关键词：
D O I：
10.1016/j.xpro.2020.100152
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Lentiviral vectors are an ideal gene-delivery system for large gene-editing tools, such as the clustered regularly interspaced short palindromic repeat (CRISPR)Cas9 system, due to their high packaging capacity and broad tropism. Here, we present a calcium phosphate-based protocol for lentiviral production and concentration for in vitro and in vivo use. This revised procedure has been optimized to ensure high viral titers and transduction efficiency and is scalable to meet specific production needs.
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页数：16
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