Microsystem for the immunomagnetic detection of Escherichia coli O157:H7

被引:3
|
作者
Mujika, M. [1 ]
Arana, S. [1 ]
Castano, E. [1 ]
Tijero, M. [2 ]
Vilares, R. [2 ]
Ruano-Lopez, J. M. [2 ]
Cruz, A. [3 ]
Sainz, L. [3 ]
Berganza, J. [3 ]
机构
[1] CEIT IK4 CIC microGUNE, San Sebastian, Spain
[2] IKERLAN IK4 CIC microGUNE, Arrasate Mondragon 20500, Spain
[3] GAIKER IK4, Ctr Technol, Zamudio, Spain
关键词
D O I
10.1002/pssa.200778126
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
A hand held microsystem has been designed for the immunomagnetic detection and quantification of the pathogen Escherichia coli O157:H7 in food and clinical samples (see abstract figure). In this work a technology that facilitates the integration of 3D microfluidic networks with any microfabricated biosensor to manufacture a Lab On a Chip has been developed. With this aim, the design of the microfluidic circuitry, the sensing film optimization, the packaging needed to carry out the assays in a safe and straightforward way and, finally, the development of the biological protocols involved in the measurements have been completed. [GRAPHICS] Photograph of a packaged biosensor ready to be tested. (C) 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码:1478 / 1483
页数:6
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