Expeditious synthesis of a new hexasaccharide using transglycosylation reaction catalyzed by Bacillus (1→3),(1→4)-β-D-glucan 4-glucanohydrolase

被引：15
|
作者：
Viladot, JL
Stone, B
Driguez, H
Planas, A
机构：
[1] Univ Ramon Llull, Inst Quim Sarria, Lab Bioquim, Barcelona 08017, Spain
[2] La Trobe Univ, Dept Biochem, Melbourne, Vic 3083, Australia
[3] Univ Grenoble 1, CNRS, Ctr Rech Macromol Vegetales, F-38041 Grenoble, France
来源：
CARBOHYDRATE RESEARCH | 1998年 / 311卷 / 1-2期
关键词：
enzymatic synthesis; transglycosylation; (1 -> 3),(1 -> 4)-beta-glucanase; glycosyl fluoride; hexasaccharide;
D O I：
10.1016/S0008-6215(98)00212-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Enzymatic hydrolysis of barley (1-->3),(1-->4)-beta-D-glucan using a recombinant (1-->3),(1-->4)-beta-glucanase from Bacillus licheniformis gives Glc beta 4Glc beta 3Glc isolated after acetylation in 49% yield. Conventional treatment produced the corresponding beta-fluoride which was carefully de-O-acetylated. A transglycosylation reaction with this substrate, catalyzed by the title enzyme, gave Glc beta 4Glc beta 3Glc beta 4Glc beta 4Glc beta 3Glc in 20% yield. (C) 1998 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：95 / 99
页数：5
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