Expeditious synthesis of a new hexasaccharide using transglycosylation reaction catalyzed by Bacillus (1→3),(1→4)-β-D-glucan 4-glucanohydrolase

被引：15

作者：

Viladot, JL

Stone, B

Driguez, H

Planas, A

机构：

[1] Univ Ramon Llull, Inst Quim Sarria, Lab Bioquim, Barcelona 08017, Spain

[2] La Trobe Univ, Dept Biochem, Melbourne, Vic 3083, Australia

[3] Univ Grenoble 1, CNRS, Ctr Rech Macromol Vegetales, F-38041 Grenoble, France

来源：

CARBOHYDRATE RESEARCH | 1998年 / 311卷 / 1-2期

关键词：

enzymatic synthesis; transglycosylation; (1 -> 3),(1 -> 4)-beta-glucanase; glycosyl fluoride; hexasaccharide;

D O I：

10.1016/S0008-6215(98)00212-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Enzymatic hydrolysis of barley (1-->3),(1-->4)-beta-D-glucan using a recombinant (1-->3),(1-->4)-beta-glucanase from Bacillus licheniformis gives Glc beta 4Glc beta 3Glc isolated after acetylation in 49% yield. Conventional treatment produced the corresponding beta-fluoride which was carefully de-O-acetylated. A transglycosylation reaction with this substrate, catalyzed by the title enzyme, gave Glc beta 4Glc beta 3Glc beta 4Glc beta 4Glc beta 3Glc in 20% yield. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：95 / 99

页数：5

共 50 条

[1] Molecular weight, structure and shape of oat (1→3),(l → 4)-β-D-glucan fractions obtained by enzymatic degradation with (1→4)-β-D-glucan 4-glucanohydrolase from Trichoderma reesei
Roubroeks, JP
Andersson, R
Mastromauro, DI
Christensen, BE
Åman, P
CARBOHYDRATE POLYMERS, 2001, 46 (03) : 275 - 285
[2] PRIMARY STRUCTURE OF THE (1-]3,1-]4)-BETA-D-GLUCAN 4-GLUCANOHYDROLASE FROM BARLEY ALEURONE
FINCHER, GB
LOCK, PA
MORGAN, MM
LINGELBACH, K
WETTENHALL, REH
MERCER, JFB
BRANDT, A
THOMSEN, KK
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (07) : 2081 - 2085
[3] Contribution of a disulfide bridge to the stability of 1,3-1,4-beta-D-glucan 4-glucanohydrolase from Bacillus licheniformis
Pons, J
Planas, A
Querol, E
PROTEIN ENGINEERING, 1995, 8 (09): : 939 - 945
[4] IFCC methods for the measurement of catalytic concentration of enzymes part 9. IFCC METHOD FOR α-amylase (1-4-α-D-Glucan 4-Glucanohydrolase, EC 3.2.1.1)
Lorentz K.
Indian Journal of Clinical Biochemistry, 1997, 12 (1) : 1 - 24
[5] In vitro synthesis of a crystalline (1 → 3,1 → 4)-β-D-glucan by a mutated (1 → 3,1 → 4)-β-D-glucanase from Bacillus
Faijes, M
Ima, T
Bulone, V
Planas, A
BIOCHEMICAL JOURNAL, 2004, 380 : 635 - 641
[6] CHROMOSOMAL MAPPING OF THE GLB-1 LOCUS ENCODING (1-]3),(1-]4)-BETA-D-GLUCAN 4-GLUCANOHYDROLASE EI IN BARLEY
MACLEOD, LC
LANCE, RCM
BROWN, AHD
JOURNAL OF CEREAL SCIENCE, 1991, 13 (03) : 291 - 298
[7] ANALYSIS OF GLYCAN STRUCTURES OF BARLEY (1-3,1-4)-BETA-D-GLUCAN 4-GLUCANOHYDROLASE ISOENZYME EII
HARTHILL, JE
THOMSEN, KK
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 1995, 33 (01) : 9 - 18
[8] CRYSTAL-STRUCTURE OF BACILLUS-LICHENIFORMIS 1,3-1,4-BETA-D-GLUCAN 4-GLUCANOHYDROLASE AT 1.8 ANGSTROM RESOLUTION
HAHN, M
PONS, J
PLANAS, A
QUEROL, E
HEINEMANN, U
FEBS LETTERS, 1995, 374 (02) : 221 - 224
[9] ENZYMIC DISSOCIATION OF ZEA SHOOT CELL-WALL POLYSACCHARIDES .2. DISSOCIATION OF (1-]3),(1-]4)-BETA-D-GLUCAN BY PURIFIED (1-]3),(1-]4)-BETA-D-GLUCAN 4-GLUCANOHYDROLASE FROM BACILLUS-SUBTILIS
KATO, Y
NEVINS, DJ
PLANT PHYSIOLOGY, 1984, 75 (03) : 745 - 752
[10] Understanding the mechanism of (1,3;1,4)-β-D-glucan synthesis in cereals
Doblin, Monika
Ho, Yin Ying
Oehme, Daniel
Bain, Melissa
Ford, Kristina
van de Meene, Allison
Lampugnani, Edwin
Bacic, Antony
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2017, 253

← 1 2 3 4 5 →