A Three-Dimensional Tracking Method with the Self-Calibration Functions of Coaxiality and Magnification for Single Fluorescent Nanoparticles

被引：0

作者：

Mao, Shuai ^{[1
]}

Shen, Jin ^{[1
]}

Wang, Ya-Jing ^{[1
]}

Liu, Wei ^{[1
]}

Pan, Jinfeng ^{[1
]}

机构：

[1] Shandong Univ Technol, Sch Elect & Elect Engn, Zibo 255000, Peoples R China

来源：

APPLIED SCIENCES-BASEL | 2020年 / 10卷 / 01期

基金：

中国国家自然科学基金; 中国博士后科学基金;

关键词：

single fluorescent nanoparticles; 3D tracking; microscopical measurement; CONFOCAL MICROSCOPE; PARTICLE TRACKING; SYSTEM;

D O I：

10.3390/app10010131

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Featured Application A self-calibrating variable magnification three-dimensional (3D) tracking system for single fluorescent nanoparticles is proposed. The proposed tracking system had the self-calibration functions of coaxiality and magnification; thus, it could accurately track the adopted fluorescent nanoparticles in 3D through zoom microscopical measurements. Abstract A self-calibrating variable magnification three-dimensional (3D) tracking system for single fluorescent nanoparticles is proposed. The system was based on astigmatic micro-imaging and has a simple configuration incorporating a dual-spot position detection unit ray transfer matrix. By analyzing this matrix and utilizing the beam vector measurement of a dual-spot position detection unit, it was demonstrated that the proposed tracking system had the self-calibration functions of coaxiality and magnification; thus, it could accurately track the adopted fluorescent nanoparticles through zoom microscopical measurements in 3D. The available measurements of the proposed system and accuracy were experimentally validated.

引用

页数：13

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