Production of IL-1 beta and IL-6 by adherent spleen cells after the stimulation with lipopolysaccharides from Salmonella enteritidis strain

被引：2
|
作者：
Kollarova, Z
Rosocha, J
Mikula, I
机构：
[1] University of Veterinary Medicine, Dept. of Microbiology and Immunology
来源：
IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY | 1996年 / 18卷 / 03期
关键词：
D O I：
10.3109/08923979609052745
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Three different procedures were used to isolate lipopolysaccharides from the Salmonella enteritidis strain 477 : phenol - water extraction with ethanol precipitation (LPS 1), phenol - water extraction with methanol precipitation (LPS 2) and FPLC purification (LPS 1/1). Production of interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6) was observed in the supernatants of adherent spleen cells of BALB/c mice after the stimulation and cultivation of the cells. The quantity of IL-1 beta and IL-6 depended on the method of LPS isolation. The highest level of IL-1 beta was recorded at LPS 2, and of IL-6 at the stimulation of cells by means of LPS 1.
引用
收藏
页码：433 / 441
页数：9
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