Production of IL-1 beta and IL-6 by adherent spleen cells after the stimulation with lipopolysaccharides from Salmonella enteritidis strain

被引:2
|
作者
Kollarova, Z
Rosocha, J
Mikula, I
机构
[1] University of Veterinary Medicine, Dept. of Microbiology and Immunology
关键词
D O I
10.3109/08923979609052745
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Three different procedures were used to isolate lipopolysaccharides from the Salmonella enteritidis strain 477 : phenol - water extraction with ethanol precipitation (LPS 1), phenol - water extraction with methanol precipitation (LPS 2) and FPLC purification (LPS 1/1). Production of interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6) was observed in the supernatants of adherent spleen cells of BALB/c mice after the stimulation and cultivation of the cells. The quantity of IL-1 beta and IL-6 depended on the method of LPS isolation. The highest level of IL-1 beta was recorded at LPS 2, and of IL-6 at the stimulation of cells by means of LPS 1.
引用
收藏
页码:433 / 441
页数:9
相关论文
共 50 条
  • [1] Stimulation of IL-1 beta and IL-6 production in human dental pulp cells by peptidoglycans from carious lesion microorganisms
    Matsushima, K
    Ohbayashi, E
    Hosoda, S
    Shibata, Y
    Yamazaki, M
    Abiko, Y
    DENTIN/PULP COMPLEX, 1996, : 310 - 312
  • [2] IL-6 and IL-1 synergistically enhanced the production of MMPs from synovial cells by up-regulating IL-6 production and IL-1 receptor I expression
    Suzuki, Miho
    Hashizume, Misato
    Yoshida, Hiroto
    Shiina, Masashi
    Mihara, Masahiko
    CYTOKINE, 2010, 51 (02) : 178 - 183
  • [3] IL-1 STIMULATES IL-6 PRODUCTION IN ENDOTHELIAL-CELLS
    SIRONI, M
    BREVIARIO, F
    PROSERPIO, P
    BIONDI, A
    VECCHI, A
    VANDAMME, J
    DEJANA, E
    MANTOVANI, A
    JOURNAL OF IMMUNOLOGY, 1989, 142 (02): : 549 - 553
  • [4] IL-1 beta stabilizes IL-6 mRNA in human astrocytoma cells
    Anneleen, Spooren
    Guy, Haegeman
    Sarah, Gerlo
    JOURNAL OF NEUROIMMUNOLOGY, 2010, 228 (1-2) : 22 - 23
  • [5] Lemongrass effects on IL-1 and IL-6 production by macrophages
    Sforcin, J. M.
    Amaral, J. T.
    Fernandes, A., Jr.
    Sousa, J. P. B.
    Bastos, J. K.
    NATURAL PRODUCT RESEARCH, 2009, 23 (12) : 1151 - 1159
  • [6] INTERLEUKIN-6 (IL-6) PRODUCTION BY HUMAN MONONUCLEAR-CELLS AND COMPARISON TO IL-1 ALPHA,BETA AND TNF
    SCHINDLER, R
    ENDRES, S
    GHORBANI, R
    DINARELLO, CA
    LYMPHOKINE RESEARCH, 1988, 7 (03): : 311 - 311
  • [7] Interleukin-6 (IL-6) is secreted from the brain after intracerebroventricular injection of IL-1 beta in rats
    Romero, LI
    Kakucska, I
    Lechan, RM
    Reichlin, S
    AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 1996, 270 (03) : R518 - R524
  • [8] ANCA stimulates IL-1 beta and IL-6 production from TNF-alpha primed neutrophils
    Myers, CR
    ODonoghue, DJ
    Brenchley, PEC
    KIDNEY INTERNATIONAL, 1997, 52 (01) : 276 - 277
  • [9] IL-1 beta and IL-6 expression in the optic nerves from patients with AIDS
    Heller, KB
    Khan, I
    Lin, XH
    Gu, XZ
    Sadun, AA
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 1997, 38 (04) : 1834 - 1834
  • [10] IL-1 AND IL-6 INDUCE EXPRESSION OF IL-6 IN HUMAN VASCULAR ENDOTHELIAL-CELLS
    HEINRICH, MC
    PHAM, TQ
    MCCOY, SL
    WILKINSON, BM
    RATHBUN, RK
    HEFENEIDER, SH
    BAGBY, GC
    CLINICAL RESEARCH, 1989, 37 (01): : A155 - A155