A Molecular Study on Hepatitis E Virus (HEV) in Pigs in Bulgaria

被引:4
|
作者
Palombieri, Andrea [1 ]
Tsachev, Ilia [2 ]
Sarchese, Vittorio [1 ]
Fruci, Paola [1 ]
Di Profio, Federica [1 ]
Pepovich, Roman [3 ]
Baymakova, Magdalena [4 ]
Marsilio, Fulvio [1 ]
Martella, Vito [5 ]
Di Martino, Barbara [1 ]
机构
[1] Univ Teramo, Fac Vet Med, I-64100 Teramo, Italy
[2] Trakia Univ, Fac Vet Med, Dept Microbiol Infect & Parasit Dis, Stara Zagora 6000, Bulgaria
[3] Univ Forestry, Fac Vet Med, Dept Infect Pathol Hyg Technol & Control Foods An, Sofia 1797, Bulgaria
[4] Mil Med Acad, Dept Infect Dis, Sofia 1606, Bulgaria
[5] Univ Aldo Moro Bari, Dept Vet Med, I-70010 Valenzano, Italy
关键词
hepatitis E virus (HEV); RNA; genotype; 3; pigs; Bulgaria; INFECTION;
D O I
10.3390/vetsci8110267
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Information on hepatitis E virus (HEV) strains circulating in animal reservoirs in Bulgaria is currently lacking. Herein, by screening HEV seropositive sera obtained from Bulgarian swine and wild boars, viral RNA was detected at high prevalence rate (28.2%) in industrial pigs. Sequence analysis of the partial polymerase (RdRp) region revealed the highest genetic correlation with HEVs of genotype (Gt) 3 identified in French and Dutch patients. For three such strains, a 700-bp fragment of the open reading frame 2 gene was generated. On phylogenetic analysis, the Bulgarian strains clustered tightly (93.8-98.3% nt) with human and animal HEVs classified within the Gt3 subtype c.
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页数:7
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