Detection of Chlamydophila abortus in ovine milk by Immunomagnetic separation-polymerase chain reaction

被引：0
|
作者：
Öngör, H [1 ]
Çetinkaya, B [1 ]
Açik, MN [1 ]
Karahan, M [1 ]
Bulut, H [1 ]
机构：
[1] Univ Firat, Fac Vet Med, Dept Microbiol, TR-23119 Elazig, Turkey
来源：
JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH | 2004年 / 51卷 / 01期
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中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The present study was carried out to investigate the presence of Chlamydophila abortus, the causative agent of ovine enzootic abortion, in milk samples collected from sheep flocks with and without the history of abortion in Eastern Turkey by means of immunomagnetic separation (IMS) in conjunction with the polymerase chain reaction (PCR). A total number of 201 milk samples collected from 10 flocks with abortion and four flocks without abortion were tested. In the analysis of the milk samples by IMS-PCR, correct amplification was obtained with three (1.5%) samples originating from one flock with abortion. In the digestion of PCR positive products by AluI, restriction profiles observed in all three samples were determined to be the same as C. abortus S26/3.
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页码：43 / 45
页数：3
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