A novel thin film urea biosensor based on copolymer poly(N-3-aminopropylpyrrole-co-pyrrole) film

被引：40

作者：

Rajesh ^{[1
]}

Bisht, V ^{[1
]}

Takashima, W ^{[1
]}

Kaneto, K ^{[1
]}

机构：

[1] Kyushu Inst Technol, Grad Sch Life Sci & Syst Engn, Kitakyushu, Fukuoka 8080196, Japan

来源：

SURFACE & COATINGS TECHNOLOGY | 2005年 / 198卷 / 1-3期

基金：

日本学术振兴会;

关键词：

biosensor; polymer; peptide bonding; covalent immobilization; enzyme;

D O I：

10.1016/j.surfcoat.2004.10.022

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The enzyme, urease (Urs), was covalently immobilized to an electrochemically prepared novel copolymer poly(N-3-aminopropylpyrrole-co-pyrrole) (PAPCP) film onto an indium-tin-oxide (ITO)-coated glass plate for the fabrication of urea biosensor. The urease enzyme retains its bioactivity when being immobilized on N-substituted pyrrole polymer film by covalent bonding. The biosensor was characterized with infrared and scanning electron microscopy. Potentiometeric response was measured as a function of urea concentration in Tris-HCl buffer (pH 7.0). The electrode showed a linear response range of 6.3 x 10(-6) to 4.07 x 10(-4) M urea. The biosensor exhibited a response time of 25-50 s, reaching to a 95% steady state potential value, and was stable for about 2 months. (c) 2004 Elsevier B.V. All rights reserved.

引用

页码：231 / 236

页数：6

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