A novel thin film urea biosensor based on copolymer poly(N-3-aminopropylpyrrole-co-pyrrole) film

被引：40
|
作者：
Rajesh [1 ]
Bisht, V [1 ]
Takashima, W [1 ]
Kaneto, K [1 ]
机构：
[1] Kyushu Inst Technol, Grad Sch Life Sci & Syst Engn, Kitakyushu, Fukuoka 8080196, Japan
来源：
SURFACE & COATINGS TECHNOLOGY | 2005年 / 198卷 / 1-3期
基金：
日本学术振兴会;
关键词：
biosensor; polymer; peptide bonding; covalent immobilization; enzyme;
D O I：
10.1016/j.surfcoat.2004.10.022
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
The enzyme, urease (Urs), was covalently immobilized to an electrochemically prepared novel copolymer poly(N-3-aminopropylpyrrole-co-pyrrole) (PAPCP) film onto an indium-tin-oxide (ITO)-coated glass plate for the fabrication of urea biosensor. The urease enzyme retains its bioactivity when being immobilized on N-substituted pyrrole polymer film by covalent bonding. The biosensor was characterized with infrared and scanning electron microscopy. Potentiometeric response was measured as a function of urea concentration in Tris-HCl buffer (pH 7.0). The electrode showed a linear response range of 6.3 x 10(-6) to 4.07 x 10(-4) M urea. The biosensor exhibited a response time of 25-50 s, reaching to a 95% steady state potential value, and was stable for about 2 months. (c) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：231 / 236
页数：6
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