Electrochemical DNA biosensor for the detection of Trichoderma harzianum based on a gold electrode modified with a composite membrane made from an ionic liquid, ZnO nanoparticles and chitosan, and by using acridine orange as a redox indicator

被引：22

作者：

Siddiquee, Shafiquzzaman ^{[1
,5
]}

Yusof, Nor Azah ^{[1
,2
]}

Salleh, Abu Bakar ^{[2
,3
]}

Tan, Soon Guan ^{[3
]}

Abu Bakar, Fatimah ^{[2
,4
]}

机构：

[1] Univ Putra Malaysia, Fac Sci, Dept Chem, Serdang 43400, Selangor, Malaysia

[2] Univ Putra Malaysia, Inst Biosci, Serdang 43400, Selangor, Malaysia

[3] Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Cell & Mol Biol, Serdang 43400, Selangor, Malaysia

[4] Univ Putra Malaysia, Fac Food Sci & Technol, Dept Food Sci, Serdang 43400, Selangor, Malaysia

[5] Univ Malaysia Sabah, Biotechnol Res Inst, Kota Kinabalu, Sabah, Malaysia

来源：

MICROCHIMICA ACTA | 2011年 / 172卷 / 3-4期

关键词：

DNA electrochemical biosensor; DNA hybridization; ZnO nanopaticles; Ionic liquid; Chitosan; Trichoderma harzianum; WALLED CARBON NANOTUBES; ETHIDIUM-BROMIDE; PASTE ELECTRODE; ACID; IMMOBILIZATION; SENSORS;

D O I：

10.1007/s00604-010-0498-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An electrochemical DNA biosensor was developed that is based on a gold electrode modified with a nano-composite membrane made from an ionic liquid, ZnO nanoparticles and chitosan. A single-stranded DNA probe was immobilized on this electrode. Acridine orange was used as the hybridization probe for monitoring the hybridization of the target DNA. The biosensor was capable of detecting target DNA in the concentration range from 1.0x10-14 to 1.8x10-4 mol L-1, with a detection limit of 1.0x10-15 mol L-1. The approach towards constructing a DNA biosensor allows studies on the hybridization even with crude DNA fragments and also to analyze sample obtained from real samples. The results show that the DNA biosensor has the potential for sensitive detection of a specific sequence of the Trichoderma harzianum gene and provides a quick, sensitive and convenient method for the study of microorganisms.

引用

页码：357 / 363

页数：7

共 21 条

[21] Disposable electrochemical immunosensor for myeloperoxidase based on the indium tin oxide electrode modified with an ionic liquid composite film containing gold nanoparticles, poly(o-phenylenediamine) and carbon nanotubes
Bei Liu
Lingsong Lu
Qiong Li
Guoming Xie
Microchimica Acta, 2011, 173 : 513 - 520

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