Genetic relationships of cattle breeds assessed by PCR-RFLP of the bovine mitochondrial DNA D-loop region

被引：2

作者：

Yoon, DH ^{[1
]}

Lee, HK ^{[1
]}

Oh, SJ ^{[1
]}

Hong, KC ^{[1
]}

Jeon, GJ ^{[1
]}

Kong, HS ^{[1
]}

Lee, JH ^{[1
]}

机构：

[1] Natl Livestock Res Inst, Div Anim Genom & Bioinformat, Suwon 441350, South Korea

来源：

ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES | 2005年 / 18卷 / 10期

关键词：

Bos Taurus; Bos indicus; mitochondrial DNA; PCR-RFLP; genetic distance;

D O I：

10.5713/ajas.2005.1368

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

To investigate the genetic relationships among various cattle breeds, bovine mtDNA D-loop region was used in 411 animals of 18 cattle breeds, including 8 Asian Bos Taurus, 7 European Bos taurus, 1 Asian Bos indicus, and 2 African Bos indicus. The size of amplified PCR products from mtDNA D-loop region was 964 by and the products were digested by 15 different restriction enzymes. Two different band patterns were identified in eight restriction enzymes (BstXI, Hae III, Msp I, Apa I, Taq I, Alu I, BamH I, EcoN I) and the rest of restriction enzymes showed more than 3 different band patterns among which Apo I and MspR9 resulted in 7 different restriction patterns. The genotypes, number of haplotype, effective number of haplotype, and degree of heterozygosity were analyzed. Based on all the PCR-RFLP data, different haplotypes were constructed and analyzed for calculating genetic distances between these breeds using Nei's unbiased method and constructing a phylogenetic tree.

引用

页码：1368 / 1374

页数：7

共 50 条

[21] The genetic variability of two Egyptian chicken breeds based on mitochondrial DNA D-loop region patterns
Haitham, Yacoub A.
Moataz, Badwy, I
[J]. RESEARCH JOURNAL OF BIOTECHNOLOGY, 2019, 14 (11): : 9 - 14
[22] Haplotyping of mitochondrial DNA in the D-loop region by PCR: Forensic application
Pai, CY
Chou, SL
Tang, TK
Wei, YH
Yang, CH
[J]. JOURNAL OF THE FORMOSAN MEDICAL ASSOCIATION, 1997, 96 (02) : 73 - 82
[23] Genetic Characterization of the Aceh Cattle Using Phenotypic, Mitochondrial DNA of D-Loop Region and Microsatellite DNA Analyses
Abdullah, M. A. N.
Martojo, H.
Noor, R. R.
Solihin, D. D.
[J]. REPRODUCTION IN DOMESTIC ANIMALS, 2012, 47 : 15 - 17
[24] Differentiation of cattle species in beef by PCR-RFLP of mitochondrial and satellite DNA
Verkaar, ELC
Nijman, IJ
Boutaga, K
Lenstra, JA
[J]. MEAT SCIENCE, 2002, 60 (04) : 365 - 369
[25] Diversity of D-loop mitochondrial DNA (mtDNA) sequence in Bali and Sumba Ongole cattle breeds
Jakaria, J.
Musyaddad, T.
Rahayu, S.
Muladno, M.
Sumantri, C.
[J]. JOURNAL OF THE INDONESIAN TROPICAL ANIMAL AGRICULTURE, 2019, 44 (04) : 335 - 345
[26] 6个近交系小鼠线粒体DNA D-Loop区PCR-RFLP分析
戴纪刚
魏泓
肖颖彬
[J]. 陆军军医大学学报, 1999, (10) : 709 - 711
[27] The Analysis on the GD of Cultured Japanese Flounder Based on PCR-RFLP of MtDNA D-loop
Qiao Zhiyi
Dong Shi
[J]. AGRICULTURAL REFORMATION AND RURAL PROGRESSING, 2010, : 282 - +
[28] 4个群体鲢mtDNA D-loop的PCR-RFLP分析
王淞
曹晓霞
谷口顺彦
董仕
[J]. 淡水渔业, 2010, 40 (04) : 3 - 9
[29] 3个群体泥鳅mtDNA D-loop的PCR-RFLP分析
荣朝振
祖国掌
胡建华
孙守旗
孙棠丽
[J]. 水生态学杂志, 2011, 32 (04) : 91 - 96
[30] Allele-specific PCR typing and sequencing of the mitochondrial D-loop region in four layer breeds
Harumi, Takashi
Sano, Akiko
Minematsu, Takeo
Naito, Mitsuru
[J]. ANIMAL SCIENCE JOURNAL, 2011, 82 (02) : 223 - 226

← 1 2 3 4 5 →