One-day pulsed-field gel electrophoresis protocol for rapid determination of emetic Bacillus cereus isolates

被引：5

作者：

Kaminska, Paulina S. ^{[1
]}

Fiedoruk, Krzysztof ^{[2
]}

Jankowska, Dominika ^{[1
]}

Mahillon, Jacques ^{[3
]}

Nowosad, Karol ^{[4
]}

Drewicka, Ewa ^{[1
]}

Zambrzycka, Monika ^{[1
]}

Swiecicka, Izabela ^{[1
,5
]}

机构：

[1] Univ Bialystok, Dept Microbiol, PL-15950 Bialystok, Poland

[2] Med Univ Bialystok, Dept Microbiol, Bialystok, Poland

[3] Catholic Univ Louvain, Lab Food & Environm Microbiol, Louvain La Neuve, Belgium

[4] Marie Curie Sklodowska Univ, Dept Genet & Microbiol, Lublin, Poland

[5] Univ Bialystok, Lab Appl Microbiol, PL-15950 Bialystok, Poland

来源：

ELECTROPHORESIS | 2015年 / 36卷 / 7-8期

关键词：

Bacillus cereus; Emetic isolates; PFGE; Rapid protocol; THURINGIENSIS; WEIHENSTEPHANENSIS; OUTBREAK; STRAINS; DIVERSITY;

D O I：

10.1002/elps.201400552

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacillus cereus, the Gram-positive and spore-forming ubiquitous bacterium, may cause emesis as the result of food intoxication with cereulide, a heat-stable emetic toxin. Rapid determination of cereulide-positive B. cereus isolates is of highest importance due to consequences of this intoxication for human health and life. Here we present a 1-day pulsed-field gel electrophoresis for emetic B. cereus isolates, which allows rapid and efficient determination of their genomic relatedness and helps determining the source of intoxication in case of outbreaks caused by these bacilli.

引用

页码：1051 / 1054

页数：4

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