Digital microfluidic three-dimensional cell culture and chemical screening platform using alginate hydrogels

被引：20

作者：

George, Subin M. ^{[1
]}

Moon, Hyejin ^{[1
]}

机构：

[1] Univ Texas Arlington, Dept Mech & Aerosp Engn, Arlington, TX 76019 USA

来源：

BIOMICROFLUIDICS | 2015年 / 9卷 / 02期

基金：

美国国家科学基金会;

关键词：

IMMOBILIZATION;

D O I：

10.1063/1.4918377

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Electro wetting-on-dielectric (EWOD) digital microfluidics (DMF) can be used to develop improved chemical screening platforms using 3-dimensional (3D) cell culture. Alginate hydrogels are one common method by which a 3D cell culture environment is created. This paper presents a study of alginate gelation on EWOD DMF and investigates designs to obtain uniform alginate hydrogels that can be repeatedly addressed by any desired liquids. A design which allows for gels to be retained in place during liquid delivery and removal without using any physical barriers or hydrophilic patterning of substrates is presented. A proof of concept screening platform is demonstrated by examining the effects of different concentrations of a test chemical on 3D cells in alginate hydrogels. In addition, the temporal effects of the various chemical concentrations on different hydrogel posts are demonstrated, thereby establishing the benefits of an EWOD DMF 3D cell culture and chemical screening platform using alginate hydrogels. (C) 2015 AIP Publishing LLC.

引用

页数：13

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