Effect of enzymes adsorption on enzymatic hydrolysis of coffee silverskin: Kinetic characterization and validation

被引：6
|
作者：
Russo, M. E. [1 ]
Procentese, A. [2 ]
Montagnaro, F. [3 ]
Marzocchella, A. [4 ]
机构：
[1] Ist Sci & Tecnol Energia & Mobilita Sostenibil, Consiglio Nazl Ric, Ple V Tecchio 80, I-80125 Naples, Italy
[2] Tech Univ Denmark, Dept Biotechnol & Biomed, DK-2800 Lyngby, Denmark
[3] Univ Napoli Federico II, Dipartimento Sci Chim, Complesso Univ Monte SantAngelo, I-80126 Naples, Italy
[4] Univ Napoli Federico II, Dipartimento Ingn Chim Mat & Prod Ind, Ple V Tecchio 80, I-80125 Naples, Italy
来源：
BIOCHEMICAL ENGINEERING JOURNAL | 2022年 / 180卷
关键词：
Enzymatic hydrolysis; Kinetics; Adsorption; Coffee silverskin;
D O I：
10.1016/j.bej.2022.108364
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The use of waste lignocellulose feedstock for sugar-based biorefineries is attracting the interest of the scientific and industrial communities. The aim is to develop efficient sustainable processes to produce fuels and chemicals via the biotechnological route. However, the rational design of processes aimed at fermentable sugar recovery from lignocellulosic wastes asks for reliable quantitative data of a wide spectrum of biomass. Waste biomasses from food industries are among the most studied potential carbon sources. In the present study, the kinetics of enzymatic hydrolysis of coffee silverskin has been characterized. Coffee silverskin is one of the most abundant fractions of the coffee industry waste and it has been already used for saccharification purposes. The experimental investigation provided kinetic parameters of a semi-mechanistic model of glucose production rate from coffee silverskin catalyzed by a commercial cellulase cocktail. In addition, the adsorption of the enzymes on the solid biomass substrate has been characterized according to a Langmuir type model. The effect of enzymes adsorption on cellulose conversion and the process dynamics have been highlighted by validation tests. The optimization of biocatalyst use has been provided via a two-step hydrolysis procedure. The developed procedure can be successfully applied in the future to several biomasses to describe a wide range of possible substrates.
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页数：7
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