Topoisomerase Inhibition, Nucleolytic and Electrolytic Contribution on DNA Binding Activity Exerted by Biological Active Analogue of Coordination Compounds

被引：30
|
作者：
Patel, Mohan N. [1 ]
Bhatt, Bhupesh S. [1 ]
Dosi, Promise A. [1 ]
机构：
[1] Sardar Patel Univ, Dept Chem, Vallabh Vidyanagar 388120, Gujarat, India
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2012年 / 166卷 / 08期
关键词：
Gyrase inhibition; DNA binding free energy; Radical scavenging agents; Thermal denaturation study; Hydrolytic cleavage; QUINOLONE ANTIBACTERIAL AGENTS; CALF THYMUS DNA; METAL-COMPLEXES; COPPER(II) COMPLEXES; CLEAVAGE; LIGAND; DRUGS; ACID; MONONUCLEAR; CHEMISTRY;
D O I：
10.1007/s12010-012-9623-x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The neutral mononuclear copper complexes with the quinolone antibacterial drug ciprofloxacin and bipyridine derivatives have been synthesized and characterized. Complexes were screened for their antibacterial activity against three Gram((-)) and two Gram((+)) bacteria, and study suggests inhibition of gyrase activity by metal complexes as the possible mechanism. The nucleolytic activity of adducts was carried out on double stranded pUC19 DNA using gel electrophoresis in the presence of radical scavenging agents that suggest hydrolytic cleavage mechanism for plasmid DNA.
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页码：1949 / 1968
页数：20
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