Study of Optimal Real-time Polymerase Chain Reaction Chip by Comparison of Coating Reflective Materials

被引：1

作者：

Hwang, Ji-Soo ^{[1
,2
]}

Kim, Bo-Jun ^{[1
,2
]}

Kim, Jong-Dae ^{[1
,2
]}

Kim, Yu-Seop ^{[1
,2
]}

Song, Hye-Jeong ^{[1
,2
]}

Park, Chan-Young ^{[1
,2
]}

机构：

[1] Hallym Univ, Dept Convergence Software, 1 Hallimdaehak Gil, Chuncheon Si 24252, Gangwon Do, South Korea

[2] Hallym Univ, BioIT Res Ctr, 1 Hallimdaehak Gil, Chuncheon Si 24252, Gangwon Do, South Korea

来源：

SENSORS AND MATERIALS | 2019年 / 31卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

real-time polymerase chain reaction; fluorescence detection; relative gap; microfluidic channel; PCR chip; lab-on-a-chip; PCR;

D O I：

10.18494/SAM.2019.2101

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

The real-time polymerase chain reaction (RT-PCR) test chip employed in previous experiments used a matte black printed circuit board (PCB) substrate printed with a white silk legend on a heating pattern. However, when analyzing the fluorescence detection results, the chip needed to be replaced with a coating material with a high reflection efficiency because of a large variation in brightness. Therefore, in this work, chips using a PCB substrate coated with various kinds of materials are manufactured, and the relative gains of these chips are compared to select a chip showing the most optimal reflection efficiency. As a result, the reflection efficiency of the PCR chip using a silver-coated PCB substrate was found to be the best.

引用

页码：437 / 445

页数：9

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