Comparison of Real-time Polymerase Chain Reaction Results by Different Illumination Methods

被引：8
|
作者：
Hwang, Ji-Soo [1 ,2 ]
Kim, Jong-Dae [2 ,3 ]
Kim, Yu-Seop [2 ,3 ]
Song, Hye-Jeong [2 ,3 ]
Park, Chan-Young [2 ,3 ]
机构：
[1] Hallym Univ, Dept Comp Engn, 1 Hallimdaehak Gil, Chuncheon Si 24252, Gangwon Do, South Korea
[2] Hallym Univ, Dept Convergence Software, 1 Hallimdaehak Gil, Chuncheon Si 24252, Gangwon Do, South Korea
[3] Hallym Univ, BioIT Res Ctr, 1 Hallimdaehak Gil, Chuncheon Si 24252, Gangwon Do, South Korea
来源：
SENSORS AND MATERIALS | 2018年 / 30卷 / 03期
基金：
新加坡国家研究基金会;
关键词：
polymerase chain reaction; fluorescence detection test; PCR chip; lab on a chip; illumination method; real-time PCR; quantitative analysis;
D O I：
10.18494/SAM.2018.1760
中图分类号：
TH7 [仪器、仪表];
学科分类号：
0804 ; 080401 ; 081102 ;
摘要：
In this paper, we introduce the diagonal illumination method and the vertical illumination method of the real-time polymerase chain reaction (PCR) system and compare the fluorescence intensity change patterns of the two methods using images captured with a digital single lens reflex (DSLR) camera during the PCR process. To overcome the problems of diagonal illumination, the PCR was performed by vertical illumination. The results of the PCR process with the proposed vertical illumination method were similar to those of the diagonal illumination method.
引用
收藏
页码：397 / 402
页数：6
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