Application of combined immunohistochemical panel of AMACR(P504S)/p63 cocktail, cytokeratin 5 and D2-40 to atypical glands in prostatic needle biopsy

Various immunohistochemical studies using cocktail antibodies to elucidate atypical glands in prostatic biopsy specimens have been previously tried. However, there is scanty information on combined cocktail antibodies and other basal cell markers. We investigated the utility of an immunohistochemical panel of AMACR/p63, cytokeratin 5(CK5) and D2-40 for atypical glands in twenty lesions of fourteen patients obtained from prostatic needle biopsy specimens. The final diagnosis of all lesions, including 13 adenocarcinoma demonstrating AMACR+/basal cell(p63, CK5 and D2-40)-pattern, 5 benign lesions noting AMACR-/basal cell+ pattern, and 2 high grade PIN with AMACR+/basal cell+ pattern, were resolved. The immunohistochemical panel of AMACR(P504S)/p63 cocktail, CK5 and D2-40 is useful in deciding the final diagnosis for atypical gland foci in the prostatic needle biopsy specimens and is helpful in the reduction of opportunity of further followup or re-biopsy.