Nontargeted metabolite profiling evaluation in patients with myelodysplastic syndrome based on LC-QTOF-MS

The present study aimed to explore the abnormal metabolites of patients with myelodysplastic syndrome (MDS) using liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry (LC-QTOF-MS). Both serum and urine samples from 20 patients with MDS (MDS group) and 20 healthy volunteers (normal control, NC group) were collected, and the metabolite profiles were analyzed systematically using LC-QTOF-MS. Untargeted substantial differences were detected between the metabolite signatures of the MDS and NC groups using partial least-squares discriminant analysis. A total of 12 metabolites in the amino acid and nucleotide metabolic pathways presented abnormal levels in the serum. Abnormalities were also detected in 7 metabolites in the urine. Thus, these identified abnormal metabolites suggested a substantial metabolite disorder in patients with MDS, which would be valuable in further investigation and diagnosis of MDS.