Symptoms of fruit blotch were noticed on roselle (Hibiscus sabdariffa) in Relau, Penang and Karangan, Kedah. The aims of this study were to isolate and identify the suspected causal pathogen and to evaluate its pathogenicity towards the host. Two isolates of Fusarium sp. were isolated from the blotched fruits of H. sabdariffa and morphologically identified. Sequences analysis based on translation elongation factor (TEF1-alpha) and mitochondrial small subunit ribosomal DNA (mtSSU rDNA) showed that both isolates had 99.53% identity to AF333934 and 100% to AF333941, Fusarium concentricum. Phylogenetic analysis demonstrated that both isolates were in the same clade with the reference isolates of F. concentricum from GenBank, supported by 97% bootstrap value. The isolates were pathogenic toward H. sabdariffa, exhibiting typical blotch symptoms on the inoculated fruits. This is the first report of fruit blotch disease caused by F. concentricum on H. sabdariffa in Malaysia.
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Shandong Agr Univ, Coll Plant Protect, Collaborat Innovat Ctr Fruit & Vegetable Qual & E, Tai An 271018, Shandong, Peoples R ChinaShandong Agr Univ, Coll Plant Protect, Collaborat Innovat Ctr Fruit & Vegetable Qual & E, Tai An 271018, Shandong, Peoples R China
Zhang, X. P.
Xia, J. W.
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Shandong Agr Univ, Coll Plant Protect, Collaborat Innovat Ctr Fruit & Vegetable Qual & E, Tai An 271018, Shandong, Peoples R ChinaShandong Agr Univ, Coll Plant Protect, Collaborat Innovat Ctr Fruit & Vegetable Qual & E, Tai An 271018, Shandong, Peoples R China
Xia, J. W.
Liu, J. K.
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Chiping Plant Protecting Stn, Liaocheng 252100, Shandong, Peoples R ChinaShandong Agr Univ, Coll Plant Protect, Collaborat Innovat Ctr Fruit & Vegetable Qual & E, Tai An 271018, Shandong, Peoples R China
Liu, J. K.
Zhao, D.
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Shandong Agr Univ, Coll Plant Protect, Collaborat Innovat Ctr Fruit & Vegetable Qual & E, Tai An 271018, Shandong, Peoples R ChinaShandong Agr Univ, Coll Plant Protect, Collaborat Innovat Ctr Fruit & Vegetable Qual & E, Tai An 271018, Shandong, Peoples R China
Zhao, D.
Kong, L. G.
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Shandong Agr Univ, Coll Plant Protect, Collaborat Innovat Ctr Fruit & Vegetable Qual & E, Tai An 271018, Shandong, Peoples R ChinaShandong Agr Univ, Coll Plant Protect, Collaborat Innovat Ctr Fruit & Vegetable Qual & E, Tai An 271018, Shandong, Peoples R China
Kong, L. G.
Zhu, X. P.
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Shandong Agr Univ, Coll Plant Protect, Collaborat Innovat Ctr Fruit & Vegetable Qual & E, Tai An 271018, Shandong, Peoples R ChinaShandong Agr Univ, Coll Plant Protect, Collaborat Innovat Ctr Fruit & Vegetable Qual & E, Tai An 271018, Shandong, Peoples R China
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Hubei Univ, Coll Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430032, Peoples R ChinaHubei Univ, Coll Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430032, Peoples R China
Yang, Wenjuan
Zhang, Lu
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Hubei Univ, Coll Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430032, Peoples R ChinaHubei Univ, Coll Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430032, Peoples R China
Zhang, Lu
Zhang, Honghao
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Hubei Univ, Coll Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430032, Peoples R ChinaHubei Univ, Coll Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430032, Peoples R China
Zhang, Honghao
Li, Hao
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Tobacco Res Inst Hubei Prov, Wuhan 430033, Peoples R ChinaHubei Univ, Coll Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430032, Peoples R China
Li, Hao
Yang, Yong
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Hubei Univ, Coll Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430032, Peoples R ChinaHubei Univ, Coll Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430032, Peoples R China
Yang, Yong
Yu, Jun
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Tobacco Res Inst Hubei Prov, Wuhan 430033, Peoples R ChinaHubei Univ, Coll Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430032, Peoples R China
Yu, Jun
Xiang, Haibo
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Hubei Univ, Coll Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430032, Peoples R ChinaHubei Univ, Coll Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430032, Peoples R China