Rapid detection of tenuazonic acid in cereal and fruit juice using a lateral-flow immunochromatographic assay strip

被引：16
|
作者：
Kong, Dezhao [1 ]
Liu, Liqiang [1 ]
Song, Shanshan [1 ]
Zheng, Qiankun [1 ]
Wu, Xiaoling [1 ]
Kuang, Hua [1 ]
机构：
[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
来源：
FOOD AND AGRICULTURAL IMMUNOLOGY | 2017年 / 28卷 / 06期
关键词：
Tenuazonic acid; monoclonal antibody; lateral-flow immunochromatographic assay strip; cereal; fruit juice; ALTERNARIA MYCOTOXINS; TOMATO PRODUCTS; IC-ELISA; MILK; FOOD; CHROMATOGRAPHY; TOXICITY; APPLE;
D O I：
10.1080/09540105.2017.1337085
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A lateral-flow immunochromatographic assay (ICA) strip was developed for determination of tenuazonic acid (TeA) using both semi-quantitative and quantitative methods. The ICA strip was based on produced anti-TeA monoclonal antibody, and could be used for TeA analysis in cereal and fruit juice, with visual limits of detection of 1600ng/g and 400ng/mL, and cut-off values of 6400ng/g and 3200ng/mL, respectively. The calculated limits of detection values were 126.4ng/g and 65.3ng/mL for wheat and apple juice samples, respectively. The recovery rates ranged from 90% to 129%. In summary, the developed strip was effective for TeA detection and would be suitable for on-site detection and rapid screening of samples.
引用
收藏
页码：1293 / 1303
页数：11
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