Rapid detection of tenuazonic acid in cereal and fruit juice using a lateral-flow immunochromatographic assay strip

被引：16

作者：

Kong, Dezhao ^{[1
]}

Liu, Liqiang ^{[1
]}

Song, Shanshan ^{[1
]}

Zheng, Qiankun ^{[1
]}

Wu, Xiaoling ^{[1
]}

Kuang, Hua ^{[1
]}

机构：

[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

来源：

FOOD AND AGRICULTURAL IMMUNOLOGY | 2017年 / 28卷 / 06期

关键词：

Tenuazonic acid; monoclonal antibody; lateral-flow immunochromatographic assay strip; cereal; fruit juice; ALTERNARIA MYCOTOXINS; TOMATO PRODUCTS; IC-ELISA; MILK; FOOD; CHROMATOGRAPHY; TOXICITY; APPLE;

D O I：

10.1080/09540105.2017.1337085

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A lateral-flow immunochromatographic assay (ICA) strip was developed for determination of tenuazonic acid (TeA) using both semi-quantitative and quantitative methods. The ICA strip was based on produced anti-TeA monoclonal antibody, and could be used for TeA analysis in cereal and fruit juice, with visual limits of detection of 1600ng/g and 400ng/mL, and cut-off values of 6400ng/g and 3200ng/mL, respectively. The calculated limits of detection values were 126.4ng/g and 65.3ng/mL for wheat and apple juice samples, respectively. The recovery rates ranged from 90% to 129%. In summary, the developed strip was effective for TeA detection and would be suitable for on-site detection and rapid screening of samples.

引用

页码：1293 / 1303

页数：11

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