Detection of plum pox virus in root and bark samples from dormant plum trees

被引：6

作者：

Adams, AN ^{[1
]}

Guise, CM ^{[1
]}

Crossley, SJ ^{[1
]}

机构：

[1] Hort Res Int, Maidstone ME19 6BJ, Kent, England

来源：

17TH INTERNATIONAL SYMPOSIUM ON VIRUS AND VIRUS-LIKE DISEASES OF TEMPERATE FRUIT CROPS: FRUIT TREE DISEASES, VOLS 1 AND 2 | 1998年 / 472期

关键词：

ELISA; polymerase chain reaction; PCR; nursery; Prunus;

D O I：

10.17660/ActaHortic.1998.472.58

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

An immune-capture polymerase chain reaction (IC-PCR) protocol and ELISA were compared for their effectiveness in detecting plum pox virus (PPV) in dormant plum material. Although the IC-PCR was about 1000 times more sensitive than ELISA, PPV was detected by ELISA in 71-80% of bark samples collected in December. January and March from pot-grown rootstock trees inoculated with PPV the previous March, compared to 85-86% detection in the same samples by IC-PCR. In similar samples from one year old shoots taken from infected branches of orchard trees, 66-81% were positive by ELISA compared to 81-87% by IC-PCR. With bulked samples taken from the fibrous roots of the pot-grown trees, PPV was detected in 92-100% of samples by IC-PCR in winter compared to only 38-65% by ELISA; the extra sensitivity of IC-PCR may offer a reliable method for assessing infection in young trees and rootstocks lifted from nursery rows.

引用

页码：469 / 474

页数：6

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