Detection of plum pox virus in root and bark samples from dormant plum trees

被引：6

作者：

Adams, AN ^{[1
]}

Guise, CM ^{[1
]}

Crossley, SJ ^{[1
]}

机构：

[1] Hort Res Int, Maidstone ME19 6BJ, Kent, England

来源：

17TH INTERNATIONAL SYMPOSIUM ON VIRUS AND VIRUS-LIKE DISEASES OF TEMPERATE FRUIT CROPS: FRUIT TREE DISEASES, VOLS 1 AND 2 | 1998年 / 472期

关键词：

ELISA; polymerase chain reaction; PCR; nursery; Prunus;

D O I：

10.17660/ActaHortic.1998.472.58

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

An immune-capture polymerase chain reaction (IC-PCR) protocol and ELISA were compared for their effectiveness in detecting plum pox virus (PPV) in dormant plum material. Although the IC-PCR was about 1000 times more sensitive than ELISA, PPV was detected by ELISA in 71-80% of bark samples collected in December. January and March from pot-grown rootstock trees inoculated with PPV the previous March, compared to 85-86% detection in the same samples by IC-PCR. In similar samples from one year old shoots taken from infected branches of orchard trees, 66-81% were positive by ELISA compared to 81-87% by IC-PCR. With bulked samples taken from the fibrous roots of the pot-grown trees, PPV was detected in 92-100% of samples by IC-PCR in winter compared to only 38-65% by ELISA; the extra sensitivity of IC-PCR may offer a reliable method for assessing infection in young trees and rootstocks lifted from nursery rows.

引用

页码：469 / 474

页数：6

共 50 条

[1] Plum pox virus detection in dormant plum trees by PCR and ELISA
Adams, AN
Guise, CM
Crossley, SJ
[J]. PLANT PATHOLOGY, 1999, 48 (02) : 240 - 244
[2] INVESTIGATIONS ON THE DETECTION OF PLUM POX VIRUS IN PLUM TREES
GRUNTZIG, M
FUCHS, E
KEGLER, H
[J]. ARCHIV FUR PHYTOPATHOLOGIE UND PFLANZENSCHUTZ-ARCHIVES OF PHYTOPATHOLOGY AND PLANT PROTECTION, 1986, 22 (06): : 441 - 449
[3] Plum trees resistant to the plum pox virus
Ravelonandro, M
[J]. BIOFUTUR, 1997, (172) : 53 - 54
[4] DETECTION OF DIFFERENT TYPES OF RESISTANCE TO PLUM POX VIRUS IN PLUM TREES
KEGLER, H
BAUER, E
GRUNTZIG, M
FUCHS, E
VERDEREVSKAJA, TD
BIVOL, TF
[J]. ARCHIV FUR PHYTOPATHOLOGIE UND PFLANZENSCHUTZ-ARCHIVES OF PHYTOPATHOLOGY AND PLANT PROTECTION, 1985, 21 (05): : 339 - 346
[5] Relationship between Plum pox virus and Plum bark split virus (ACLSV) in plum
Minoiu, N
Vladianu, D
Ravelonandro, M
Oprean, I
Stegerean, P
[J]. PROCEEDINGS OF THE XIXTH INTERNATIONAL SYMPOSIUM ON VIRUS AND VIRUS-LIKE DISEASES OF TEMPERATE FRUIT CROPS: FRUIT TREE DISEASES, 2004, (657): : 265 - 267
[6] Detection of plum pox virus infection in selection plum trees using spectral imaging
Angelova, Liliya
Stoev, Antoniy
Borisova, Ekaterina
Avramov, Latchezar
[J]. 19TH INTERNATIONAL CONFERENCE AND SCHOOL ON QUANTUM ELECTRONICS: LASER PHYSICS AND APPLICATIONS, 2017, 10226
[7] A PCR MEMBRANE SPOT ASSAY FOR THE DETECTION OF PLUM POX VIRUS-RNA IN BARK OF INFECTED TREES
KORSCHINECK, I
HIMMLER, G
SAGL, R
STEINKELLNER, H
KATINGER, HWD
[J]. JOURNAL OF VIROLOGICAL METHODS, 1991, 31 (2-3) : 139 - 146
[8] RESISTANCE OF SOME PLUM CULTIVARS AND INDIVIDUAL TREES TO PLUM POX (SHARKA) VIRUS
SUTIC, D
RANKOVIC, M
[J]. AGRONOMIE, 1981, 1 (08): : 617 - 622
[9] Hypersensitivity of plum genotypes to plum pox virus
Kegler, H
Grüntzig, M
Fuchs, E
Rankovic, M
Ehrig, F
[J]. JOURNAL OF PHYTOPATHOLOGY, 2001, 149 (3-4) : 213 - 218
[10] PLUM POX VIRUS
CONTI, M
[J]. NATURE, 1984, 308 (5956) : 221 - 221

← 1 2 3 4 5 →