A TICT based two-photon fluorescent probe for bisulfite anion and its application in living cells

被引：57
|
作者：
Yu, Shenglong [1 ]
Yang, Xiuli [2 ]
Shao, Zhonglong [1 ]
Feng, Yan [1 ]
Xi, Xinguo [2 ]
Shao, Rong [2 ]
Guo, Qingxiang [1 ,3 ]
Meng, Xiangming [1 ,2 ]
机构：
[1] Anhui Univ, Dept Chem, Hefei 230601, Peoples R China
[2] Yancheng Inst Technol, Jiangsu Collaborat Innovat Ctr Ecol Bldg Mat & En, Yancheng 224051, Peoples R China
[3] Univ Sci & Technol China, Dept Chem, Hefei 230026, Peoples R China
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2016年 / 235卷
基金：
中国国家自然科学基金;
关键词：
TICT; Two-photon; Fluorescent probe; Coumarin; Bisulfite anion; SULFUR-DIOXIDE DERIVATIVES; TURN-ON PROBE; SELECTIVE DETECTION; SO2; DERIVATIVES; SULFITE; MITOCHONDRIA; WATER; ABSORPTION;
D O I：
10.1016/j.snb.2016.05.099
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A two-photon fluorescent probe (DMPCA) for the detection of bisulfite anion in water was developed based on twisted-intramolecular-charge-transfer (TICT) mechanism. Fluorescence intensity of DMPCA increased dramatically (65 fold) upon the addition of HS O-3(-). The two-photon absorption cross-section of DMPCA was found to be 725 GM at 700 nm upon the addition of HSO3-. The fluorescent probe showed an excellent selectivity for bisulfite anion over other anions. The nucleophilic addition reaction mechanism with aldehyde was confirmed with NMR and MALDI-TOF. The TICT detection mechanism was confirmed by viscosity-dependent fluorescence spectra and time resolved fluorescence spectra. Moreover, DMPCA was successfully applied to the detection of bisulfite anion in HeLa cells with low cytotoxicity under two-photon excitation. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码：362 / 369
页数：8
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