Effects of bioprocessed soybean meal and nucleotide supplementation on growth, physiology and histomorphology in largemouth bass, Micropterus salmoides, juveniles

In this 8-week feeding trial, the effects of nucleotide (N) supplementation (at 0.05%) were compared in diets with conventional soybean meal (CSBM or CSBM + N) versus bioprocessed SBM (BSBM or BSMB + N) on largemouth bass, Micropterus salmoides, juveniles. A total of five isonitrogenous and isolipidic diets were formulated, with the control diet being fishmeal-based. Growth, feeding efficiency, proximate composition, hepatic expression of genes involved in lipid metabolism and growth as well as liver/intestinal histopathology were assessed. Results showed that growth was significantly higher in fish fed the control diet, but there was no significant effect of SBM type or nucleotide supplementation on growth, feeding efficiency, or proximate composition. Hepatic expression of growth hormone (GH), insulin-like growth factor I (IGF-I), superoxide dismutase (SOD), fatty acid synthase (FASN) and cholesterol 7 alpha-hydroxylase (CYP7A1) were unaffected by the diets. Tumor necrosis factor alpha (TNF-alpha) and transforming growth factor beta (TGF-beta) were significantly downregulated and upregulated, respectively, in the SBM-based treatments compared with the control. The intestinal villi were significantly shorter and wider in fish fed the CSBM diet compared to the other treatments. The villi height and width were similar between the control and those fed the BSMB + N diet. It may be possible that the unaffected growth by nucleotides were due to an insufficient dose and/or undisrupted nucleotide synthesis due to being cultured under good conditions. Meanwhile, the unaffected growth in the SBM treatment could indicate a tolerance of M. salmoides to plant proteins and associated antinutritional factors. Nevertheless, BSBM and/or nucleotides appeared to mitigate some adverse effects of dietary SBM to the intestinal histomorphology in M. salmoides.