In Vivo Metabolite Profiling of a Purified Ellagitannin Isolated from Polygonum capitatum in Rats

被引：11
|
作者：
Ma, Jing-Yi [1 ]
Zhou, Xuelin [2 ]
Fu, Jie [1 ]
He, Chi-Yu [1 ]
Feng, Ru [1 ]
Huang, Min [1 ]
Shou, Jia-Wen [1 ]
Zhao, Zhen-Xiong [1 ]
Li, Xiao-Yang [1 ]
Zhang, Luye [1 ]
Chen, Yang-Chao [2 ]
Wang, Yan [1 ]
机构：
[1] Chinese Acad Med Sci, Inst Mat Med, State Key Lab Bioact Subst & Funct Nat Med, Beijing 100050, Peoples R China
[2] Chinese Univ Hong Kong, Sch Biomed Sci, Fac Med, Shatin, Hong Kong, Peoples R China
来源：
MOLECULES | 2016年 / 21卷 / 09期
基金：
中国国家自然科学基金;
关键词：
FR429; ellagitannin; metabolite profiling; LC; MSn-IT-TOF; POTENTIAL ANTITUMOR ELLAGITANNIN; ELLAGIC ACID; IDENTIFICATION; ANTIOXIDANT; INHIBITION; GERANIIN; URINARY; FR429;
D O I：
10.3390/molecules21091110
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Ellagitannin is a common compound in food and herbs, but there are few detailed studies on the metabolism of purified ellagitannins. FR429 is a purified ellagitannin with antitumor potential, which is from Polygonum capitatum Buch.-Ham.ex D. Don. The present study was designed to investigate the metabolic profiles of FR429 in rats in vivo. Using liquid chromatography coupled to ion trap time-of-flight mass spectrometry (LC/MSn-IT-TOF), total eight metabolites were found in rat bile and urine after intravenous administration of FR429, but could not be detected in plasma. These metabolites were ellagic acid, mono-methylated FR429, ellagic acid methyl ether glucuronide, ellagic acid methyl ether diglucuronide, ellagic acid dimethyl ether glucuronide, and ellagic acid dimethyl ether diglucuronide. It was concluded that methylation and subsequent glucuronidation were the major metabolic pathways of FR429 in rats in vivo. This is the first report on the in vivo metabolism of the purified ellagitannin in rats.
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页数：10
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