Structure of a polysaccharide from a Rhizobium species containing 2-deoxy-β-D-arabino-hexuronic acid

被引：56
|
作者：
Guentas, L
Pheulpin, P
Michaud, P
Heyraud, A
Gey, C
Courtois, B
Courtois, J
机构：
[1] Univ Picardie, Inst Univ Technol, Dept Gen Biol, Lab Polysaccharides Microbiens & Vegetaux, F-80025 Amiens, France
[2] CNRS, Ctr Rech Macromol Vegetales, UPR 5301, F-38041 Grenoble, France
来源：
CARBOHYDRATE RESEARCH | 2001年 / 332卷 / 02期
关键词：
extracellular polysaccharide; Rhizobium sp; structural analysis; 2-deoxy-arabino-D-hexuronic acid;
D O I：
10.1016/S0008-6215(01)00080-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The structure of the extracellular polysaccharide (EPS) produced by the Rhizobium sp. B strain isolated from atypical nodules on alfalfa has been determined using a combination of chemical and physical techniques (methylation analysis, high pH-anion exchange chromatography (HPAEC), mass spectrometry and 1-D and 2-D NMR spectroscopy). As opposed to the EPS from other strains of Rhizobium, the EPS from the sp. B strain contains D-Glc together with L-Rha and 2-deoxy-D-arabino -hexuronic acid. It is a polymer of a repeating unit having the following structure: --> 4)-beta -D-Glcp -(1 --> 4)-alpha -L-Rhap-(1 --> 3)-beta -D-Glcp -(1 --> 4)-2-deoxy-beta -D-GlcpA-(l --> The polysaccharide also contains 0.6 O-acetyl groups per sugar which have not been located. (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：167 / 173
页数：7
相关论文
共 50 条
  • [31] Synthesis of aryl-2-deoxy-D-lyxo/arabino-hexopyranosides from 2-deoxy-1-thioglycosides
    Paul, Somak
    Jayaraman, Narayanaswamy
    CARBOHYDRATE RESEARCH, 2007, 342 (10) : 1305 - 1314
  • [32] SYNTHESIS AND REACTIONS OF SOME 2-DIAZO DERIVATIVES OF 2-DEOXY-D-ARABINO-HEXONIC ACID
    HORTON, D
    PHILIPS, KD
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1971, (MAR-A): : 5 - &
  • [33] Solution structure by NMR and molecular dynamics of a duplex containing a guanine opposite a N-(2-deoxy-β-D-erythro-pentofuranosyl)formamide lesion
    Maufrais, C
    Fazakerley, GV
    Cadet, J
    Boulard, Y
    BIOCHEMISTRY, 2000, 39 (18) : 5614 - 5621
  • [34] THE CRYSTAL-STRUCTURE OF "2-DEOXY-BETA-D-ARABINO-HEXOPYRANOSE AT -150-DEGREES
    MALUSZYNSKA, H
    RUBLE, JR
    JEFFREY, GA
    CARBOHYDRATE RESEARCH, 1981, 97 (02) : 199 - 204
  • [35] STRUCTURAL STUDIES ON A NEW POLYSACCHARIDE, CONTAINING D-RIBURONIC ACID, FROM RHIZOBIUM-MELILOTI IFO-13336
    AMEMURA, A
    HISAMATSU, M
    GHAI, SK
    HARADA, T
    CARBOHYDRATE RESEARCH, 1981, 91 (01) : 59 - 65
  • [36] THERMAL-DEGRADATION OF 2-DEOXY-D-ARABINO-HEXONIC ACID AND 3-DEOXY-D-RIBO-HEXONO-1,4-LACTONE
    SHAFIZADEH, F
    ZONGLAI, Y
    CARBOHYDRATE RESEARCH, 1975, 42 (01) : 39 - 53
  • [37] Structure of a polysaccharide from the lipopolysaccharide of Vibrio vulnificus clinical isolate YJ016 containing 2-acetimidoylamino-2-deoxy-L-galacturonic acid
    Senchenkova, Sof'ya N.
    Shashkov, Alexander S.
    Knirel, Yuriy A.
    Esteve, Consuelo
    Alcaide, Elena
    Merino, Susana
    Tomas, Juan M.
    CARBOHYDRATE RESEARCH, 2009, 344 (08) : 1009 - 1013
  • [38] STRUCTURE OF THE EXTRACELLULAR POLYSACCHARIDE FROM RHODOCOCCUS SP CONTAINING D-LYXO-HEXULOSONIC ACID
    VINOGRADOV, EV
    KNIREL, YA
    SHASHKOV, AS
    GORIN, SE
    VUSTINA, TF
    SOYFER, VS
    ESIPOV, SE
    LISAK, LV
    KOCHETKOV, NK
    BIOORGANICHESKAYA KHIMIYA, 1988, 14 (09): : 1214 - 1223
  • [39] Structure of the α-polysaccharide of Proteus serogroup O34 containing 2-acetamido-2-deoxy-α-D-galactosyl phosphate
    Perepelov, AV
    Kolodziejska, K
    Kondakova, AN
    Wykrota, M
    Knirel, YA
    Sidorczyk, Z
    Rozalski, A
    CARBOHYDRATE RESEARCH, 2004, 339 (12) : 2145 - 2149
  • [40] SYNTHESIS OF NATURAL 3-DEOXY-D-ARABINO-HEPTULOSONIC ACID FROM 2,3-ANHYDRO-2-CHLORO-4,5-6,7-DI-O-CYCLOHEXYLIDENE-D-ARABINO-METHYLHEPTONATE
    KORNILOV, VI
    BICHEROVA, II
    TURIK, SV
    ZHDANOV, YA
    BIOORGANICHESKAYA KHIMIYA, 1988, 14 (07): : 938 - 943
12345