Haeamtang induces apoptosis of colon cancer HT-29 cells through activation of caspase-3

被引：8
|
作者：
Moon, Phil-Dong
Koo, Hyun-Na
Jeong, Hyun-Ja
Na, Ho-Jeong
Kim, Su-Jin
Hwang, Gab-Soo
Han, Na-Ra
Hong, Seung-Heon
Kim, Hyung-Min
Um, Jae-Young
机构：
[1] Kyung Hee Univ, Coll Oriental Med, Dept Pharmacol, Oriental Med Sci Ctr, Seoul 130701, South Korea
[2] Kyung Hee Univ, Coll Oriental Med, Dept Pharmacol, Canc Prevent Mat Dev Res Ctr, Seoul 130701, South Korea
[3] Korea Res Inst Chem Technol, Div Med Sci, Taejon 305343, South Korea
[4] Wonkwang Univ, VestibuloCochlear Res Ctr, Coll Pharm, Iksan 570749, Jeonbuk, South Korea
[5] Woosuk Univ, Coll Pharm, Wonju 565701, Jeonbuk, South Korea
[6] Kunsan Natl Univ, Sch Civil & Environm Engn, Kunsan 573701, Jeonbuk, South Korea
来源：
AMERICAN JOURNAL OF CHINESE MEDICINE | 2007年 / 35卷 / 05期
关键词：
haeamtang; HT-29; cells; caspase-3; z-VAD-fmk;
D O I：
10.1142/S0192415X07005363
中图分类号：
R [医药、卫生];
学科分类号：
10 ;
摘要：
The effect of Haeamtang ( HAT) on the colon cancer HT-29 cells was investigated in this study. A water extract of HAT significantly decreased the number of HT-29 cells in a dose- and time-dependent manner as determined by a MTT assay. Flow cytometry results revealed a dose- and time-dependent increase of dead cells in HT-29 cells treated with HAT extract. The anticancer activity of the HAT extract is attributed to apoptosis induced in HT-29 cells, which was demonstrated by increased caspase-3 activity and poly-ADP-ribose polymerase fragmentation. A selective caspase inhibitor, z-VAD-fmk, inhibited the HAT-induced cell death. Taken together, these results demonstrate that HAT extract induces apoptosis in HT-29 cells.
引用
收藏
页码：897 / 909
页数：13
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