Expression of platelet-derived endothelial cell growth factor and its mRNA in uterine endometrium during the menstrual cycle

被引:30
|
作者
Fujimoto, J [1 ]
Ichigo, S [1 ]
Sakaguchi, H [1 ]
Hirose, R [1 ]
Tamaya, T [1 ]
机构
[1] Gifu Univ, Sch Med, Dept Obstet & Gynecol, Gifu 5008705, Japan
关键词
PD-ECGF; neovascularization; uterine endometrium;
D O I
10.1093/molehr/4.5.509
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Steroid hormones, e.g. progesterone and oestradiol, may be responsible for the production and expression of a variety of angiogenic growth factors present in endometrial tissue. The expression of platelet-derived endothelial cell growth factor (PD-ECGF) in neovascularization after regression of the microvessels in the endometrium was examined. PD-ECGF protein expression in the endometrium during the menstrual cycle was determined by a sandwich enzyme immunoassay. Transcription levels of PD-ECGF were measured by a quantitative reverse transcription-polymerase chain reaction (RT-PCR) Southern blot technique. The data show that levels of PD-ECGF protein and mRNA in uterine endometrium did not alter during the proliferative phase prior to ovulation. During the midcycle phase a sharp transient fall in mRNA revels accompanied by a gradual drop in protein levels was observed. After ovulation transcription of PD-ECGF recovered with a sharp increase in mRNA levels which persisted during the ovulatory phase. PD-ECGF protein levels were temporarily low after ovulation, but increased remarkably through the late secretory phase. PD-ECGF expression in the endometrium seems to be inversely correlated with oestradiol concentrations during the menstrual cycle.
引用
收藏
页码:509 / 513
页数:5
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