Characteristics and diversity of endophytic bacteria in moso bamboo (Phyllostachys edulis) based on 16S rDNA sequencing

被引:22
|
作者
Liu, Fang [1 ]
Yuan, Zongsheng [1 ]
Zhang, Xintao [1 ,3 ]
Zhang, Guofang [2 ]
Xie, Baogui [1 ]
机构
[1] Fujian Agr & Forestry Univ, Sch Life Sci, Fuzhou 350002, Fujian, Peoples R China
[2] Fujian Agr & Forestry Univ, Coll Forestry, Fuzhou 350002, Fujian, Peoples R China
[3] Nanjing Wanchen Biol Technol Co LTD, Nanjing 211299, Jiangsu, Peoples R China
关键词
Phyllostachys edulis; Endophytic bacteria; 16S rDNA; 454; pyrosequencing; Diversity; GROWTH; RICE;
D O I
10.1007/s00203-017-1397-7
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
To understand the diversity and distribution of endophytic bacteria in moso bamboo (Phyllostachys edulis), we used 16S rDNA sequencing to investigate the characteristics and diversity of endophytic bacteria in different moso bamboo tissues. After 454 pyrosequencing, we obtained 141,269 sequences from seven moso bamboo tissue samples. The taxonomic origins of unique sequences were identified using RDP classifier. The results showed that these sequences belonged to 26 bacterial orders, including the Actinomycetales, Rickettsiales, Burkholderiales, Enterobacteriales, and Rhizobiales. Among these, Enterobacteriales was widely found in all bamboo tissues. Endophytic bacterial communities differed between the moso bamboo shoot and pole. With continuous growth and development, the number of endophytic species in the moso bamboo pole increased gradually.
引用
收藏
页码:1259 / 1266
页数:8
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