Identification of Phoenix dactylifera L. varieties based on amplified fragment length polymorphism (AFLP) markers

被引：0
|
作者：
Diaz, S [1 ]
Pire, C [1 ]
Ferrer, J [1 ]
Bonete, MJ [1 ]
机构：
[1] Univ Alicante, Fac Ciencies, Dept Agr & Bioquim, E-03080 Alicante, Spain
来源：
CELLULAR & MOLECULAR BIOLOGY LETTERS | 2003年 / 8卷 / 04期
关键词：
Phoenix dactylifera; amplified fragment length polymorphism (AFLP); molecular markers; vitroplant analysis;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The amplified fragment length polymorphism (AFLP) technique was applied to identify palm varieties. Fluorescence labelled primers were used in selective amplifications and the amplified fragments were detected on capillary get electrophoresis using an automated DNA sequencer with the analysis fragment option. This is a rapid and efficient technique for detecting a large number of DNA markers on the date palm. Phoenix dactylifera L. varieties BouFegous, Medjool, and E-528 from Estacion Phoenix (Elche), Spain, were analysed, yielding a total of 310 AFLP fragments derived from five primer combinations. The process for regenerating the date palm cultivars from in vitro tissue culture should yield individuals phenotypically and genetically identical to the explant they are derived from. The AFLP markers obtained were successfully used for comparing and identifying vitroplants of palm.
引用
收藏
页码：891 / 899
页数：9
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