An aptamer based on-plate microarray for high-throughput insulin detection by MALDI-TOF MS

被引：40
|
作者：
Zhang, Xueyang
Zhu, Shaochun
Deng, Chunhui [1 ]
Zhang, Xiangmin
机构：
[1] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 21期
基金：
中国国家自然科学基金;
关键词：
ENZYME-IMMUNOASSAY; MASS-SPECTROMETRY; SERUM-PROTEINS; ASSAY; APTASENSOR; MOLECULES; GOLD;
D O I：
10.1039/c2cc17997k
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
An aptamer microarray was directly fabricated on a MALDI target plate for high-throughput insulin detection. High sensitivities were observed both in standard solutions (5 ng mL(-1), 0.86 nM) and serum sample (20 ng mL(-1), 3.4 nM). This method shows great promise in the field of biomarker detection.
引用
收藏
页码：2689 / 2691
页数：3
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