Detection of Mycobacterium avium subsp paratuberculosis in bovine faecal samples by Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) TaqMan Assay

被引：0
|
作者：
Rasool, S. [1 ]
Narang, D. [1 ]
Chandra, M. [1 ]
Sharma, N. S. [1 ]
Singh, Tejinder S. [2 ]
机构：
[1] GADVASU, COVS, Dept Vet Microbiol, Ludhiana, Punjab, India
[2] GADVASU, COVS, Dept Anim Genet & Breeding, Ludhiana, Punjab, India
来源：
TROPICAL BIOMEDICINE | 2016年 / 33卷 / 02期
关键词：
JOHNES-DISEASE; NESTED PCR; CULTURE; FECES; MILK; DNA; CATTLE; ELISA; COWS; EXTRACTION;
D O I：
暂无
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Mycobacterium avium subsp. paratuberculosis, the causative agent of Johne's disease is a degenerative chronic granulomatous disease of bovines. In the present study, quantitative real time polymerase chain reaction (qRT-PCR) using TaqMan chemistry targeting the IS900 sequence of Mycobacterium avium subsp. paratuberculosis (MAP) was employed for the molecular diagnosis of the disease in bovine faecal samples. Out of 200 bovine faecal samples processed, 7 samples were tested as positive by IS900 qRT-PCR. The sensitivity limit of detection of MAP DNA in faecal samples by qRT-PCR TaqMan assay was found to be 0.05pg. No amplification was observed in other Mycobacterial spp. viz. M. phlei, M. smegmatis, M. intracellulare and M. kansasii.
引用
收藏
页码：327 / 334
页数：8
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