共 50 条
DNA isolation, optimization of ISSR-PCR system and primers screening of Scutellaria baicalensis
被引：0
|作者：
Guo, H. B.
[1
,2
]
Huang, K. Y.
[3
]
Zhou, T. S.
[3
]
Wu, Q. H.
[3
]
Zhang, Y. J.
[1
,2
]
Liang, Z. S.
[1
,2
]
机构：
[1] NW A&F Univ, Coll Life Sci, Shaanxi Res Ctr TCM Fingerprinting, Yangling 712100, Peoples R China
[2] NW A&F Univ, Coll Life Sci, CP Lib, Yangling 712100, Peoples R China
[3] Fudan Univ, Minist Educ, Key Lab Biodivers Sci & Ecol Engn, Res Ctr Nat Med, Shanghai 200433, Peoples R China
来源：
关键词：
DNA isolation;
ISSR;
optimal PCR condition;
primer screening;
Scutellaria baicalensis;
GENETIC DIVERSITY;
POPULATIONS;
WILD;
D O I：
暂无
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
To estimate genetic diversity and to authenticate the medicinal materials of Scutellaria baicalensis Georgi, the present work including DNA isolation, optimization of PCR assay of inter-simple sequence repeat (ISSR) and primers screening were investigated. Among three DNA isolation methods, improved CTAB, improved SDS and isolation kit, the improved CTAB was the best if restricted by funding. Based on selection design and protocols of reports, the optimal ISSR-PCR action was carried out in a volume of 20 mu l containing 20 ng of DNA template, 1.0 U of Taq DNA polymerase, 1 x buffer, 200 mu M dNTPs, 0.2 mu M of primer and 2.25 mu M Mg(2+). According to this PCR system, fifteen out of one hundred primers were chosen for their high clarity and repetition.
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页码：898 / 901
页数：4
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