Lessening polygalacturonase activity in a commercial enzyme preparation by exposure to pulsed electric fields

被引：27

作者：

Giner, J

Gimeno, V

Palomes, M

Barbosa-Cánovas, GV

Martín, O

机构：

[1] Univ Lleida, CeRTA UTPV, Dept Food Technol, ETSEA, Lleida 25198, Spain

[2] Washington State Univ, Dept Biol Syst Engn, Pullman, WA 99164 USA

来源：

EUROPEAN FOOD RESEARCH AND TECHNOLOGY | 2003年 / 217卷 / 01期

关键词：

high-intensity-pulsed-electric field; kinetics; polygalacturonase; inactivation;

D O I：

10.1007/s00217-003-0688-1

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The effect of high-intensity pulsed electric field (HIPEF) treatments on polygalacturonase (PG) activity in an aqueous solution of a commercial enzyme preparation was evaluated. HIPEF treatments reduced PG activity notably. Exponentially decaying pulses of 40 and 160 mus generated by a laboratory scale device were applied in a batch processing and bipolar mode. Electric fields ranged from 5.18 to 19.39 kV/cm. The number of pulses ranged up to 400. Temperature was always below 25 degreesC. Maximum inactivation of PG activity (98%) required 32.4 ms HIPEF treatment at 10.28 kV/cm. PG activity depleted exponentially because of HIPEF treatments. The first order rate constants ranged from 32 to 590 mus(-1) and increased exponentially with electric field intensity. PG activity decreased exponentially with input electric energy density.

引用

页码：43 / 48

页数：6

共 50 条

[31] Mechanism of pulsed electric field enzyme activity change and pulsed discharge permeabilization of agricultural products
Ohshima, Takayuki
Tanino, Takanori
Guionet, Alexis
Takahashi, Katsuyuki
Takaki, Koichi
JAPANESE JOURNAL OF APPLIED PHYSICS, 2021, 60 (06)
[32] A wideband picosecond pulsed electric fields (psPEF) exposure system for the nanoporation of biological cells
Gao, Mingxiang
Xie, Yanzhao
Wang, Siqi
Shang, Sen
Zhao, Jiping
Lu, Xiaoyun
BIOELECTROCHEMISTRY, 2021, 140
[33] Electroendocytosis: Exposure of cells to pulsed low electric fields enhances adsorption and uptake of macromolecules
Antov, Y
Barbul, A
Mantsur, H
Korenstein, R
BIOPHYSICAL JOURNAL, 2005, 88 (03) : 2206 - 2223
[34] Pulsed electric fields on phenylalanine ammonia lyase activity of tomato cell culture
Gursul, Isil
Gueven, Alper
Grohmann, Anne
Knorr, Dietrich
JOURNAL OF FOOD ENGINEERING, 2016, 188 : 66 - 76
[35] Effects of Nanosecond Pulsed Electric Fields on the Activity of a Hodgkin and Huxley Neuron Model
Camera, F.
Paffi, A.
Merla, C.
Denzi, A.
Apollonio, F.
Marracino, P.
d'Inzeo, G.
Liberti, M.
2012 ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY (EMBC), 2012, : 2567 - 2570
[36] Pulsed electric fields disaggregating chlorophyll aggregates and boosting its biological activity
Zhang, Zhi-Hong
Huang, Xin
Wang, Lang-Hong
Li, Jian
Zeng, Xin-An
Gao, Xianli
FOOD RESEARCH INTERNATIONAL, 2025, 208
[37] Microbiological and enzymatic activity of bovine whole milk treated by pulsed electric fields
Sharma, Pankaj
Oey, Indrawati
Bremer, Phil
Everett, David W.
INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY, 2018, 71 (01) : 10 - 19
[38] Antimicrobial activity of suspensions and nanoemulsions of citral in combination with heat or pulsed electric fields
Pagan, E.
Berdejo, D.
Espina, L.
Garcia-Gonzalo, D.
Pagan, R.
LETTERS IN APPLIED MICROBIOLOGY, 2018, 66 (01) : 63 - 70
[39] Pulsed electric fields affect endogenous enzyme activities, respiration and biosynthesis of phenolic compounds in carrots
Lopez-Gamez, Gloria
Elez-Martinez, Pedro
Martin-Belloso, Olga
Soliva-Fortuny, Robert
POSTHARVEST BIOLOGY AND TECHNOLOGY, 2020, 168
[40] Pulsed electric fields: Processing system, microbial and enzyme inhibition, and shelf life extension of foods
Min, Seacheol
Evrendilek, Gulsun Akdemir
Zhang, Howard Q.
IEEE TRANSACTIONS ON PLASMA SCIENCE, 2007, 35 (01) : 59 - 73

← 1 2 3 4 5 →